---
_id: '67'
abstract:
- lang: eng
  text: 'Gene regulatory networks evolve through rewiring of individual components—that
    is, through changes in regulatory connections. However, the mechanistic basis
    of regulatory rewiring is poorly understood. Using a canonical gene regulatory
    system, we quantify the properties of transcription factors that determine the
    evolutionary potential for rewiring of regulatory connections: robustness, tunability
    and evolvability. In vivo repression measurements of two repressors at mutated
    operator sites reveal their contrasting evolutionary potential: while robustness
    and evolvability were positively correlated, both were in trade-off with tunability.
    Epistatic interactions between adjacent operators alleviated this trade-off. A
    thermodynamic model explains how the differences in robustness, tunability and
    evolvability arise from biophysical characteristics of repressor–DNA binding.
    The model also uncovers that the energy matrix, which describes how mutations
    affect repressor–DNA binding, encodes crucial information about the evolutionary
    potential of a repressor. The biophysical determinants of evolutionary potential
    for regulatory rewiring constitute a mechanistic framework for understanding network
    evolution.'
article_processing_charge: No
article_type: original
author:
- first_name: Claudia
  full_name: Igler, Claudia
  id: 46613666-F248-11E8-B48F-1D18A9856A87
  last_name: Igler
  orcid: 0000-0001-7777-546X
- first_name: Mato
  full_name: Lagator, Mato
  id: 345D25EC-F248-11E8-B48F-1D18A9856A87
  last_name: Lagator
- first_name: Gasper
  full_name: Tkacik, Gasper
  id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
  last_name: Tkacik
  orcid: 0000-0002-6699-1455
- first_name: Jonathan P
  full_name: Bollback, Jonathan P
  id: 2C6FA9CC-F248-11E8-B48F-1D18A9856A87
  last_name: Bollback
  orcid: 0000-0002-4624-4612
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
citation:
  ama: Igler C, Lagator M, Tkačik G, Bollback JP, Guet CC. Evolutionary potential
    of transcription factors for gene regulatory rewiring. <i>Nature Ecology and Evolution</i>.
    2018;2(10):1633-1643. doi:<a href="https://doi.org/10.1038/s41559-018-0651-y">10.1038/s41559-018-0651-y</a>
  apa: Igler, C., Lagator, M., Tkačik, G., Bollback, J. P., &#38; Guet, C. C. (2018).
    Evolutionary potential of transcription factors for gene regulatory rewiring.
    <i>Nature Ecology and Evolution</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/s41559-018-0651-y">https://doi.org/10.1038/s41559-018-0651-y</a>
  chicago: Igler, Claudia, Mato Lagator, Gašper Tkačik, Jonathan P Bollback, and Calin
    C Guet. “Evolutionary Potential of Transcription Factors for Gene Regulatory Rewiring.”
    <i>Nature Ecology and Evolution</i>. Nature Publishing Group, 2018. <a href="https://doi.org/10.1038/s41559-018-0651-y">https://doi.org/10.1038/s41559-018-0651-y</a>.
  ieee: C. Igler, M. Lagator, G. Tkačik, J. P. Bollback, and C. C. Guet, “Evolutionary
    potential of transcription factors for gene regulatory rewiring,” <i>Nature Ecology
    and Evolution</i>, vol. 2, no. 10. Nature Publishing Group, pp. 1633–1643, 2018.
  ista: Igler C, Lagator M, Tkačik G, Bollback JP, Guet CC. 2018. Evolutionary potential
    of transcription factors for gene regulatory rewiring. Nature Ecology and Evolution.
    2(10), 1633–1643.
  mla: Igler, Claudia, et al. “Evolutionary Potential of Transcription Factors for
    Gene Regulatory Rewiring.” <i>Nature Ecology and Evolution</i>, vol. 2, no. 10,
    Nature Publishing Group, 2018, pp. 1633–43, doi:<a href="https://doi.org/10.1038/s41559-018-0651-y">10.1038/s41559-018-0651-y</a>.
  short: C. Igler, M. Lagator, G. Tkačik, J.P. Bollback, C.C. Guet, Nature Ecology
    and Evolution 2 (2018) 1633–1643.
date_created: 2018-12-11T11:44:27Z
date_published: 2018-09-10T00:00:00Z
date_updated: 2026-07-15T22:30:34Z
day: '10'
ddc:
- '570'
department:
- _id: CaGu
- _id: GaTk
- _id: JoBo
doi: 10.1038/s41559-018-0651-y
ec_funded: 1
external_id:
  isi:
  - '000447947600021'
file:
- access_level: open_access
  checksum: 383a2e2c944a856e2e821ec8e7bf71b6
  content_type: application/pdf
  creator: dernst
  date_created: 2020-05-14T11:28:52Z
  date_updated: 2020-07-14T12:47:37Z
  file_id: '7830'
  file_name: 2018_NatureEcology_Igler.pdf
  file_size: 1135973
  relation: main_file
file_date_updated: 2020-07-14T12:47:37Z
has_accepted_license: '1'
intvolume: '         2'
isi: 1
issue: '10'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Submitted Version
page: 1633 - 1643
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
- _id: 2578D616-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '648440'
  name: Selective Barriers to Horizontal Gene Transfer
- _id: 251EE76E-B435-11E9-9278-68D0E5697425
  grant_number: '24573'
  name: Design principles underlying genetic switch architecture
publication: Nature Ecology and Evolution
publication_status: published
publisher: Nature Publishing Group
publist_id: '7987'
quality_controlled: '1'
related_material:
  record:
  - id: '5585'
    relation: popular_science
    status: public
  - id: '6371'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Evolutionary potential of transcription factors for gene regulatory rewiring
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 2
year: '2018'
...