--- res: bibo_abstract: - Glyphosate (N-phosphonomethyl glycine) and its commercial herbicide formulations have been shown to exert toxicity via various mechanisms. It has been asserted that glyphosate substitutes for glycine in polypeptide chains leading to protein misfolding and toxicity. However, as no direct evidence exists for glycine to glyphosate substitution in proteins, including in mammalian organisms, we tested this claim by conducting a proteomics analysis of MDA-MB-231 human breast cancer cells grown in the presence of 100 mg/L glyphosate for 6 days. Protein extracts from three treated and three untreated cell cultures were analysed as one TMT-6plex labelled sample, to highlight a specific pattern (+/+/+/−/−/−) of reporter intensities for peptides bearing true glyphosate treatment induced-post translational modifications as well as allowing an investigation of the total proteome.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Michael N. foaf_name: Antoniou, Michael N. foaf_surname: Antoniou - foaf_Person: foaf_givenName: Armel foaf_name: Nicolas, Armel foaf_surname: Nicolas foaf_workInfoHomepage: http://www.librecat.org/personId=2A103192-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Robin foaf_name: Mesnage, Robin foaf_surname: Mesnage - foaf_Person: foaf_givenName: Martina foaf_name: Biserni, Martina foaf_surname: Biserni - foaf_Person: foaf_givenName: Francesco V. foaf_name: Rao, Francesco V. foaf_surname: Rao - foaf_Person: foaf_givenName: Cristina Vazquez foaf_name: Martin, Cristina Vazquez foaf_surname: Martin bibo_doi: 10.1186/s13104-019-4534-3 bibo_volume: 12 dct_date: 2019^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/1756-0500 dct_language: eng dct_publisher: BioMed Central@ dct_title: Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells@ fabio_hasPubmedId: '31395095' ...