{"date_published":"2019-08-08T00:00:00Z","day":"08","publication_status":"published","publication":"BMC Research Notes","author":[{"first_name":"Michael N.","last_name":"Antoniou","full_name":"Antoniou, Michael N."},{"first_name":"Armel","full_name":"Nicolas, Armel","last_name":"Nicolas","id":"2A103192-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Robin","full_name":"Mesnage, Robin","last_name":"Mesnage"},{"first_name":"Martina","last_name":"Biserni","full_name":"Biserni, Martina"},{"last_name":"Rao","full_name":"Rao, Francesco V.","first_name":"Francesco V."},{"full_name":"Martin, Cristina Vazquez","last_name":"Martin","first_name":"Cristina Vazquez"}],"pmid":1,"doi":"10.1186/s13104-019-4534-3","_id":"6819","volume":12,"external_id":{"pmid":["31395095"]},"related_material":{"record":[{"status":"public","relation":"research_data","id":"9784"}]},"intvolume":"        12","language":[{"iso":"eng"}],"quality_controlled":"1","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2020-07-14T12:47:40Z","file":[{"checksum":"4a2bb7994b7f2c432bf44f5127ea3102","file_id":"6829","date_created":"2019-08-23T11:10:35Z","access_level":"open_access","relation":"main_file","file_name":"2019_BMC_Antoniou.pdf","date_updated":"2020-07-14T12:47:40Z","file_size":1177482,"content_type":"application/pdf","creator":"dernst"}],"article_number":"494","date_created":"2019-08-18T22:00:39Z","has_accepted_license":"1","citation":{"chicago":"Antoniou, Michael N., Armel Nicolas, Robin Mesnage, Martina Biserni, Francesco V. Rao, and Cristina Vazquez Martin. “Glyphosate Does Not Substitute for Glycine in Proteins of Actively Dividing Mammalian Cells.” <i>BMC Research Notes</i>. BioMed Central, 2019. <a href=\"https://doi.org/10.1186/s13104-019-4534-3\">https://doi.org/10.1186/s13104-019-4534-3</a>.","apa":"Antoniou, M. N., Nicolas, A., Mesnage, R., Biserni, M., Rao, F. V., &#38; Martin, C. V. (2019). Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells. <i>BMC Research Notes</i>. BioMed Central. <a href=\"https://doi.org/10.1186/s13104-019-4534-3\">https://doi.org/10.1186/s13104-019-4534-3</a>","ista":"Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. 2019. Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells. BMC Research Notes. 12, 494.","short":"M.N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F.V. Rao, C.V. Martin, BMC Research Notes 12 (2019).","ama":"Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells. <i>BMC Research Notes</i>. 2019;12. doi:<a href=\"https://doi.org/10.1186/s13104-019-4534-3\">10.1186/s13104-019-4534-3</a>","ieee":"M. N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F. V. Rao, and C. V. Martin, “Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells,” <i>BMC Research Notes</i>, vol. 12. BioMed Central, 2019.","mla":"Antoniou, Michael N., et al. “Glyphosate Does Not Substitute for Glycine in Proteins of Actively Dividing Mammalian Cells.” <i>BMC Research Notes</i>, vol. 12, 494, BioMed Central, 2019, doi:<a href=\"https://doi.org/10.1186/s13104-019-4534-3\">10.1186/s13104-019-4534-3</a>."},"type":"journal_article","date_updated":"2023-02-23T14:08:14Z","month":"08","title":"Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells","article_processing_charge":"No","scopus_import":1,"publisher":"BioMed Central","abstract":[{"text":"Glyphosate (N-phosphonomethyl glycine) and its commercial herbicide formulations have been shown to exert toxicity via various mechanisms. It has been asserted that glyphosate substitutes for glycine in polypeptide chains leading to protein misfolding and toxicity. However, as no direct evidence exists for glycine to glyphosate substitution in proteins, including in mammalian organisms, we tested this claim by conducting a proteomics analysis of MDA-MB-231 human breast cancer cells grown in the presence of 100 mg/L glyphosate for 6 days. Protein extracts from three treated and three untreated cell cultures were analysed as one TMT-6plex labelled sample, to highlight a specific pattern (+/+/+/−/−/−) of reporter intensities for peptides bearing true glyphosate treatment induced-post translational modifications as well as allowing an investigation of the total proteome.","lang":"eng"}],"ddc":["570"],"year":"2019","publication_identifier":{"eissn":["1756-0500"]},"oa_version":"Published Version","department":[{"_id":"LifeSc"}],"status":"public"}