article published yes Long Zhou author 3E751364-F248-11E8-B48F-1D18A9856A870000-0002-1864-8951 Leonid A Sazanov author 338D39FE-F248-11E8-B48F-1D18A9856A870000-0002-0977-7989 LeSa department V (vacuolar)/A (archaeal)-type adenosine triphosphatases (ATPases), found in archaeaand eubacteria, couple ATP hydrolysis or synthesis to proton translocation across theplasma membrane using the rotary-catalysis mechanism. They belong to the V-typeATPase family, which differs from the mitochondrial/chloroplast F-type ATP synthasesin overall architecture. We solved cryo–electron microscopy structures of the intactThermus thermophilusV/A-ATPase, reconstituted into lipid nanodiscs, in three rotationalstates and two substates. These structures indicate substantial flexibility betweenV1and Voin a working enzyme, which results from mechanical competition between centralshaft rotation and resistance from the peripheral stalks. We also describedetails of adenosine diphosphate inhibition release, V1-Votorque transmission, andproton translocation, which are relevant for the entire V-type ATPase family. AAAS2019 eng 0036-8075 1095-9203 31439765 00048246400004310.1126/science.aaw9144 3656455 https://ist.ac.at/en/news/structure-of-protein-nano-turbine-revealed/ Zhou L, Sazanov LA. 2019. Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase. Science. 365(6455), eaaw9144. Zhou, Long, and Leonid A. Sazanov. “Structure and Conformational Plasticity of the Intact Thermus Thermophilus V/A-Type ATPase.” <i>Science</i>, vol. 365, no. 6455, eaaw9144, AAAS, 2019, doi:<a href="https://doi.org/10.1126/science.aaw9144">10.1126/science.aaw9144</a>. L. Zhou, L.A. Sazanov, Science 365 (2019). Zhou, Long, and Leonid A Sazanov. “Structure and Conformational Plasticity of the Intact Thermus Thermophilus V/A-Type ATPase.” <i>Science</i>. AAAS, 2019. <a href="https://doi.org/10.1126/science.aaw9144">https://doi.org/10.1126/science.aaw9144</a>. Zhou, L., &#38; Sazanov, L. A. (2019). Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase. <i>Science</i>. AAAS. <a href="https://doi.org/10.1126/science.aaw9144">https://doi.org/10.1126/science.aaw9144</a> Zhou L, Sazanov LA. Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase. <i>Science</i>. 2019;365(6455). doi:<a href="https://doi.org/10.1126/science.aaw9144">10.1126/science.aaw9144</a> L. Zhou and L. A. Sazanov, “Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase,” <i>Science</i>, vol. 365, no. 6455. AAAS, 2019. 68592019-09-07T19:04:45Z2023-08-29T07:52:02Z