--- res: bibo_abstract: - 'Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such “simple synapses” indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate functional and morphological aspects of docking sites at simple synapses made between cerebellar parallel fibers and molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture replicas, we find that Cav2.1 channels form several clusters per active zone with about nine channels per cluster. The mean value and range of intersynaptic variation are similar for Cav2.1 cluster numbers and for functional estimates of docking-site numbers obtained from the maximum numbers of released vesicles per action potential. Both numbers grow in relation with synaptic size and decrease by a similar extent with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers were 3.15 at 2 wk (range: 1–10) and 2.03 at 4 wk (range: 1–4), whereas the mean numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1–8) and 2.37 at 4 wk (range: 1–5). These changes were accompanied by decreases of miniature current amplitude (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2), and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic transmission with development. Altogether, these results suggest a close correspondence between the number of functionally defined vesicular docking sites and that of clusters of voltage-gated calcium channels. @eng' bibo_authorlist: - foaf_Person: foaf_givenName: Takafumi foaf_name: Miki, Takafumi foaf_surname: Miki - foaf_Person: foaf_givenName: Walter foaf_name: Kaufmann, Walter foaf_surname: Kaufmann foaf_workInfoHomepage: http://www.librecat.org/personId=3F99E422-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-9735-5315 - foaf_Person: foaf_givenName: Gerardo foaf_name: Malagon, Gerardo foaf_surname: Malagon - foaf_Person: foaf_givenName: Laura foaf_name: Gomez, Laura foaf_surname: Gomez - foaf_Person: foaf_givenName: Katsuhiko foaf_name: Tabuchi, Katsuhiko foaf_surname: Tabuchi - foaf_Person: foaf_givenName: Masahiko foaf_name: Watanabe, Masahiko foaf_surname: Watanabe - foaf_Person: foaf_givenName: Ryuichi foaf_name: Shigemoto, Ryuichi foaf_surname: Shigemoto foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8761-9444 - foaf_Person: foaf_givenName: Alain foaf_name: Marty, Alain foaf_surname: Marty bibo_doi: 10.1073/pnas.1704470114 bibo_issue: '26' bibo_volume: 114 dct_date: 2017^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/00278424 dct_language: eng dct_publisher: National Academy of Sciences@ dct_title: Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses@ fabio_hasPubmedId: '28607047' ...