[{"language":[{"iso":"eng"}],"month":"07","date_published":"2017-07-25T00:00:00Z","department":[{"_id":"CaGu"}],"publisher":"eLife Sciences Publications","date_updated":"2026-09-04T22:30:14Z","isi":1,"oa":1,"file_date_updated":"2020-07-14T12:47:48Z","publist_id":"6990","_id":"704","scopus_import":"1","intvolume":"         6","oa_version":"Published Version","type":"journal_article","citation":{"mla":"Steinrück, Magdalena, and Calin C. Guet. “Complex Chromosomal Neighborhood Effects Determine the Adaptive Potential of a Gene under Selection.” <i>ELife</i>, vol. 6, e25100, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.25100\">10.7554/eLife.25100</a>.","apa":"Steinrück, M., &#38; Guet, C. C. (2017). Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.25100\">https://doi.org/10.7554/eLife.25100</a>","chicago":"Steinrück, Magdalena, and Calin C Guet. “Complex Chromosomal Neighborhood Effects Determine the Adaptive Potential of a Gene under Selection.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.25100\">https://doi.org/10.7554/eLife.25100</a>.","ama":"Steinrück M, Guet CC. Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.25100\">10.7554/eLife.25100</a>","ista":"Steinrück M, Guet CC. 2017. Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. eLife. 6, e25100.","short":"M. Steinrück, C.C. Guet, ELife 6 (2017).","ieee":"M. Steinrück and C. C. Guet, “Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017."},"status":"public","day":"25","pubrep_id":"890","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"title":"Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection","license":"https://creativecommons.org/licenses/by/4.0/","article_processing_charge":"No","abstract":[{"text":"How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the Escherichia coli chromosome. Using a dual-fluorescence reporter that allows us to distinguish gene amplifications from other up-mutations, we track in real-time adaptive changes in expression of the drug-resistance gene. We find that the relative contribution of several mutation types differs systematically between loci due to properties of neighboring genes: essentiality, expression, orientation, termination, and presence of duplicates. These properties determine rate and fitness effects of gene amplification, deletions, and mutations compromising transcriptional termination. Thus, the adaptive potential of a gene under selection is a system-property with a complex genetic basis that is specific for each chromosomal locus, and it can be inferred from detailed functional and genomic data.","lang":"eng"}],"external_id":{"isi":["000406183700001"]},"publication_status":"published","related_material":{"record":[{"status":"public","relation":"popular_science","id":"5564"},{"status":"public","id":"26","relation":"dissertation_contains"}]},"author":[{"last_name":"Steinrück","first_name":"Magdalena","full_name":"Steinrück, Magdalena","id":"2C023F40-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1229-9719"},{"last_name":"Guet","first_name":"Calin C","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","volume":6,"has_accepted_license":"1","ddc":["576"],"corr_author":"1","file":[{"creator":"system","date_created":"2018-12-12T10:12:54Z","content_type":"application/pdf","file_name":"IST-2017-890-v1+1_elife-25100-v1.pdf","relation":"main_file","checksum":"6b908b5db9f61f6820ebd7f8fa815571","access_level":"open_access","file_size":2092088,"date_updated":"2020-07-14T12:47:48Z","file_id":"4975"},{"date_created":"2018-12-12T10:12:55Z","file_name":"IST-2017-890-v1+2_elife-25100-figures-v1.pdf","content_type":"application/pdf","creator":"system","file_id":"4976","access_level":"open_access","checksum":"ca21530389b720243552678125fdba35","relation":"main_file","file_size":3428681,"date_updated":"2020-07-14T12:47:48Z"}],"quality_controlled":"1","publication_identifier":{"issn":["2050-084X"]},"doi":"10.7554/eLife.25100","publication":"eLife","year":"2017","date_created":"2018-12-11T11:48:01Z","article_number":"e25100"}]