[{"type":"journal_article","publication":"eLife","file_date_updated":"2020-07-14T12:47:48Z","language":[{"iso":"eng"}],"date_created":"2018-12-11T11:48:01Z","year":"2017","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.7554/eLife.25100","ddc":["576"],"intvolume":"         6","_id":"704","file":[{"creator":"system","date_updated":"2020-07-14T12:47:48Z","file_id":"4975","relation":"main_file","access_level":"open_access","file_size":2092088,"file_name":"IST-2017-890-v1+1_elife-25100-v1.pdf","checksum":"6b908b5db9f61f6820ebd7f8fa815571","content_type":"application/pdf","date_created":"2018-12-12T10:12:54Z"},{"date_created":"2018-12-12T10:12:55Z","file_name":"IST-2017-890-v1+2_elife-25100-figures-v1.pdf","content_type":"application/pdf","checksum":"ca21530389b720243552678125fdba35","relation":"main_file","access_level":"open_access","file_size":3428681,"creator":"system","date_updated":"2020-07-14T12:47:48Z","file_id":"4976"}],"title":"Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection","publist_id":"6990","article_number":"e25100","publication_status":"published","oa":1,"abstract":[{"text":"How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the Escherichia coli chromosome. Using a dual-fluorescence reporter that allows us to distinguish gene amplifications from other up-mutations, we track in real-time adaptive changes in expression of the drug-resistance gene. We find that the relative contribution of several mutation types differs systematically between loci due to properties of neighboring genes: essentiality, expression, orientation, termination, and presence of duplicates. These properties determine rate and fitness effects of gene amplification, deletions, and mutations compromising transcriptional termination. Thus, the adaptive potential of a gene under selection is a system-property with a complex genetic basis that is specific for each chromosomal locus, and it can be inferred from detailed functional and genomic data.","lang":"eng"}],"publication_identifier":{"issn":["2050-084X"]},"isi":1,"status":"public","date_updated":"2026-06-13T22:31:01Z","publisher":"eLife Sciences Publications","citation":{"ieee":"M. Steinrück and C. C. Guet, “Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017.","chicago":"Steinrück, Magdalena, and Calin C Guet. “Complex Chromosomal Neighborhood Effects Determine the Adaptive Potential of a Gene under Selection.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.25100\">https://doi.org/10.7554/eLife.25100</a>.","mla":"Steinrück, Magdalena, and Calin C. Guet. “Complex Chromosomal Neighborhood Effects Determine the Adaptive Potential of a Gene under Selection.” <i>ELife</i>, vol. 6, e25100, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.25100\">10.7554/eLife.25100</a>.","ama":"Steinrück M, Guet CC. Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.25100\">10.7554/eLife.25100</a>","short":"M. Steinrück, C.C. Guet, ELife 6 (2017).","apa":"Steinrück, M., &#38; Guet, C. C. (2017). Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.25100\">https://doi.org/10.7554/eLife.25100</a>","ista":"Steinrück M, Guet CC. 2017. Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. eLife. 6, e25100."},"department":[{"_id":"CaGu"}],"corr_author":"1","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_published":"2017-07-25T00:00:00Z","volume":6,"related_material":{"record":[{"relation":"popular_science","id":"5564","status":"public"},{"status":"public","id":"26","relation":"dissertation_contains"}]},"author":[{"first_name":"Magdalena","last_name":"Steinrück","orcid":"0000-0003-1229-9719","full_name":"Steinrück, Magdalena","id":"2C023F40-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C","last_name":"Guet","orcid":"0000-0001-6220-2052"}],"article_processing_charge":"No","external_id":{"isi":["000406183700001"]},"has_accepted_license":"1","pubrep_id":"890","month":"07","day":"25","quality_controlled":"1","oa_version":"Published Version","scopus_import":"1"}]