{"date_published":"2017-07-25T00:00:00Z","oa_version":"Published Version","oa":1,"abstract":[{"text":"How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the Escherichia coli chromosome. Using a dual-fluorescence reporter that allows us to distinguish gene amplifications from other up-mutations, we track in real-time adaptive changes in expression of the drug-resistance gene. We find that the relative contribution of several mutation types differs systematically between loci due to properties of neighboring genes: essentiality, expression, orientation, termination, and presence of duplicates. These properties determine rate and fitness effects of gene amplification, deletions, and mutations compromising transcriptional termination. Thus, the adaptive potential of a gene under selection is a system-property with a complex genetic basis that is specific for each chromosomal locus, and it can be inferred from detailed functional and genomic data.","lang":"eng"}],"department":[{"_id":"CaGu"}],"publisher":"eLife Sciences Publications","file":[{"file_name":"IST-2017-890-v1+1_elife-25100-v1.pdf","relation":"main_file","content_type":"application/pdf","date_updated":"2020-07-14T12:47:48Z","date_created":"2018-12-12T10:12:54Z","file_size":2092088,"checksum":"6b908b5db9f61f6820ebd7f8fa815571","creator":"system","access_level":"open_access","file_id":"4975"},{"content_type":"application/pdf","file_name":"IST-2017-890-v1+2_elife-25100-figures-v1.pdf","relation":"main_file","creator":"system","checksum":"ca21530389b720243552678125fdba35","file_size":3428681,"date_created":"2018-12-12T10:12:55Z","date_updated":"2020-07-14T12:47:48Z","access_level":"open_access","file_id":"4976"}],"article_number":"e25100","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publist_id":"6990","intvolume":"         6","day":"25","title":"Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection","date_created":"2018-12-11T11:48:01Z","language":[{"iso":"eng"}],"file_date_updated":"2020-07-14T12:47:48Z","pubrep_id":"890","publication_identifier":{"issn":["2050084X"]},"status":"public","related_material":{"record":[{"id":"5564","status":"public","relation":"popular_science"},{"relation":"dissertation_contains","status":"public","id":"26"}]},"publication":"eLife","month":"07","citation":{"mla":"Steinrück, Magdalena, and Calin C. Guet. “Complex Chromosomal Neighborhood Effects Determine the Adaptive Potential of a Gene under Selection.” <i>ELife</i>, vol. 6, e25100, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.25100\">10.7554/eLife.25100</a>.","ista":"Steinrück M, Guet CC. 2017. Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. eLife. 6, e25100.","ieee":"M. Steinrück and C. C. Guet, “Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017.","short":"M. Steinrück, C.C. Guet, ELife 6 (2017).","ama":"Steinrück M, Guet CC. Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.25100\">10.7554/eLife.25100</a>","chicago":"Steinrück, Magdalena, and Calin C Guet. “Complex Chromosomal Neighborhood Effects Determine the Adaptive Potential of a Gene under Selection.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.25100\">https://doi.org/10.7554/eLife.25100</a>.","apa":"Steinrück, M., &#38; Guet, C. C. (2017). Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.25100\">https://doi.org/10.7554/eLife.25100</a>"},"volume":6,"ddc":["576"],"scopus_import":1,"publication_status":"published","doi":"10.7554/eLife.25100","quality_controlled":"1","year":"2017","_id":"704","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-04-18T22:30:27Z","has_accepted_license":"1","author":[{"first_name":"Magdalena","id":"2C023F40-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1229-9719","last_name":"Steinrück","full_name":"Steinrück, Magdalena"},{"full_name":"Guet, Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet","orcid":"0000-0001-6220-2052","first_name":"Calin C"}]}