{"status":"public","article_number":"e25100","intvolume":"         6","oa":1,"publisher":"eLife Sciences Publications","volume":6,"corr_author":"1","publist_id":"6990","month":"07","department":[{"_id":"CaGu"}],"license":"https://creativecommons.org/licenses/by/4.0/","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"related_material":{"record":[{"relation":"popular_science","id":"5564","status":"public"},{"status":"public","relation":"dissertation_contains","id":"26"}]},"publication_identifier":{"issn":["2050-084X"]},"year":"2017","ddc":["576"],"scopus_import":"1","language":[{"iso":"eng"}],"quality_controlled":"1","publication_status":"published","abstract":[{"text":"How the organization of genes on a chromosome shapes adaptation is essential for understanding evolutionary paths. Here, we investigate how adaptation to rapidly increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance gene inserted at different positions of the Escherichia coli chromosome. Using a dual-fluorescence reporter that allows us to distinguish gene amplifications from other up-mutations, we track in real-time adaptive changes in expression of the drug-resistance gene. We find that the relative contribution of several mutation types differs systematically between loci due to properties of neighboring genes: essentiality, expression, orientation, termination, and presence of duplicates. These properties determine rate and fitness effects of gene amplification, deletions, and mutations compromising transcriptional termination. Thus, the adaptive potential of a gene under selection is a system-property with a complex genetic basis that is specific for each chromosomal locus, and it can be inferred from detailed functional and genomic data.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","pubrep_id":"890","date_created":"2018-12-11T11:48:01Z","_id":"704","file":[{"content_type":"application/pdf","file_id":"4975","file_size":2092088,"checksum":"6b908b5db9f61f6820ebd7f8fa815571","file_name":"IST-2017-890-v1+1_elife-25100-v1.pdf","creator":"system","date_updated":"2020-07-14T12:47:48Z","access_level":"open_access","date_created":"2018-12-12T10:12:54Z","relation":"main_file"},{"creator":"system","date_updated":"2020-07-14T12:47:48Z","file_name":"IST-2017-890-v1+2_elife-25100-figures-v1.pdf","checksum":"ca21530389b720243552678125fdba35","relation":"main_file","date_created":"2018-12-12T10:12:55Z","access_level":"open_access","content_type":"application/pdf","file_id":"4976","file_size":3428681}],"publication":"eLife","author":[{"id":"2C023F40-F248-11E8-B48F-1D18A9856A87","last_name":"Steinrück","first_name":"Magdalena","orcid":"0000-0003-1229-9719","full_name":"Steinrück, Magdalena"},{"full_name":"Guet, Calin C","first_name":"Calin C","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet"}],"title":"Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection","doi":"10.7554/eLife.25100","day":"25","file_date_updated":"2020-07-14T12:47:48Z","type":"journal_article","date_updated":"2025-06-19T22:30:58Z","oa_version":"Published Version","date_published":"2017-07-25T00:00:00Z","article_processing_charge":"No"}