---
_id: '704'
abstract:
- lang: eng
  text: 'How the organization of genes on a chromosome shapes adaptation is essential
    for understanding evolutionary paths. Here, we investigate how adaptation to rapidly
    increasing levels of antibiotic depends on the chromosomal neighborhood of a drug-resistance
    gene inserted at different positions of the Escherichia coli chromosome. Using
    a dual-fluorescence reporter that allows us to distinguish gene amplifications
    from other up-mutations, we track in real-time adaptive changes in expression
    of the drug-resistance gene. We find that the relative contribution of several
    mutation types differs systematically between loci due to properties of neighboring
    genes: essentiality, expression, orientation, termination, and presence of duplicates.
    These properties determine rate and fitness effects of gene amplification, deletions,
    and mutations compromising transcriptional termination. Thus, the adaptive potential
    of a gene under selection is a system-property with a complex genetic basis that
    is specific for each chromosomal locus, and it can be inferred from detailed functional
    and genomic data.'
article_number: e25100
article_processing_charge: No
author:
- first_name: Magdalena
  full_name: Steinrück, Magdalena
  id: 2C023F40-F248-11E8-B48F-1D18A9856A87
  last_name: Steinrück
  orcid: 0000-0003-1229-9719
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
citation:
  ama: Steinrück M, Guet CC. Complex chromosomal neighborhood effects determine the
    adaptive potential of a gene under selection. <i>eLife</i>. 2017;6. doi:<a href="https://doi.org/10.7554/eLife.25100">10.7554/eLife.25100</a>
  apa: Steinrück, M., &#38; Guet, C. C. (2017). Complex chromosomal neighborhood effects
    determine the adaptive potential of a gene under selection. <i>ELife</i>. eLife
    Sciences Publications. <a href="https://doi.org/10.7554/eLife.25100">https://doi.org/10.7554/eLife.25100</a>
  chicago: Steinrück, Magdalena, and Calin C Guet. “Complex Chromosomal Neighborhood
    Effects Determine the Adaptive Potential of a Gene under Selection.” <i>ELife</i>.
    eLife Sciences Publications, 2017. <a href="https://doi.org/10.7554/eLife.25100">https://doi.org/10.7554/eLife.25100</a>.
  ieee: M. Steinrück and C. C. Guet, “Complex chromosomal neighborhood effects determine
    the adaptive potential of a gene under selection,” <i>eLife</i>, vol. 6. eLife
    Sciences Publications, 2017.
  ista: Steinrück M, Guet CC. 2017. Complex chromosomal neighborhood effects determine
    the adaptive potential of a gene under selection. eLife. 6, e25100.
  mla: Steinrück, Magdalena, and Calin C. Guet. “Complex Chromosomal Neighborhood
    Effects Determine the Adaptive Potential of a Gene under Selection.” <i>ELife</i>,
    vol. 6, e25100, eLife Sciences Publications, 2017, doi:<a href="https://doi.org/10.7554/eLife.25100">10.7554/eLife.25100</a>.
  short: M. Steinrück, C.C. Guet, ELife 6 (2017).
corr_author: '1'
date_created: 2018-12-11T11:48:01Z
date_published: 2017-07-25T00:00:00Z
date_updated: 2026-07-18T22:30:13Z
day: '25'
ddc:
- '576'
department:
- _id: CaGu
doi: 10.7554/eLife.25100
external_id:
  isi:
  - '000406183700001'
file:
- access_level: open_access
  checksum: 6b908b5db9f61f6820ebd7f8fa815571
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:12:54Z
  date_updated: 2020-07-14T12:47:48Z
  file_id: '4975'
  file_name: IST-2017-890-v1+1_elife-25100-v1.pdf
  file_size: 2092088
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- access_level: open_access
  checksum: ca21530389b720243552678125fdba35
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:12:55Z
  date_updated: 2020-07-14T12:47:48Z
  file_id: '4976'
  file_name: IST-2017-890-v1+2_elife-25100-figures-v1.pdf
  file_size: 3428681
  relation: main_file
file_date_updated: 2020-07-14T12:47:48Z
has_accepted_license: '1'
intvolume: '         6'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: eLife
publication_identifier:
  issn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
publist_id: '6990'
pubrep_id: '890'
quality_controlled: '1'
related_material:
  record:
  - id: '5564'
    relation: popular_science
    status: public
  - id: '26'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Complex chromosomal neighborhood effects determine the adaptive potential of
  a gene under selection
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 6
year: '2017'
...