---
_id: '7095'
abstract:
- lang: eng
  text: BAX, a member of the BCL2 gene family, controls the committed step of the
    intrinsic apoptotic program. Mitochondrial fragmentation is a commonly observed
    feature of apoptosis, which occurs through the process of mitochondrial fission.
    BAX has consistently been associated with mitochondrial fission, yet how BAX participates
    in the process of mitochondrial fragmentation during apoptosis remains to be tested.
    Time-lapse imaging of BAX recruitment and mitochondrial fragmentation demonstrates
    that rapid mitochondrial fragmentation during apoptosis occurs after the complete
    recruitment of BAX to the mitochondrial outer membrane (MOM). The requirement
    of a fully functioning BAX protein for the fission process was demonstrated further
    in BAX/BAK-deficient HCT116 cells expressing a P168A mutant of BAX. The mutant
    performed fusion to restore the mitochondrial network. but was not demonstrably
    recruited to the MOM after apoptosis induction. Under these conditions, mitochondrial
    fragmentation was blocked. Additionally, we show that loss of the fission protein,
    dynamin-like protein 1 (DRP1), does not temporally affect the initiation time
    or rate of BAX recruitment, but does reduce the final level of BAX recruited to
    the MOM during the late phase of BAX recruitment. These correlative observations
    suggest a model where late-stage BAX oligomers play a functional part of the mitochondrial
    fragmentation machinery in apoptotic cells.
article_number: '16565'
article_processing_charge: No
article_type: original
author:
- first_name: Margaret E
  full_name: Maes, Margaret E
  id: 3838F452-F248-11E8-B48F-1D18A9856A87
  last_name: Maes
  orcid: 0000-0001-9642-1085
- first_name: J. A.
  full_name: Grosser, J. A.
  last_name: Grosser
- first_name: R. L.
  full_name: Fehrman, R. L.
  last_name: Fehrman
- first_name: C. L.
  full_name: Schlamp, C. L.
  last_name: Schlamp
- first_name: R. W.
  full_name: Nickells, R. W.
  last_name: Nickells
citation:
  ama: Maes ME, Grosser JA, Fehrman RL, Schlamp CL, Nickells RW. Completion of BAX
    recruitment correlates with mitochondrial fission during apoptosis. <i>Scientific
    Reports</i>. 2019;9. doi:<a href="https://doi.org/10.1038/s41598-019-53049-w">10.1038/s41598-019-53049-w</a>
  apa: Maes, M. E., Grosser, J. A., Fehrman, R. L., Schlamp, C. L., &#38; Nickells,
    R. W. (2019). Completion of BAX recruitment correlates with mitochondrial fission
    during apoptosis. <i>Scientific Reports</i>. Springer Nature. <a href="https://doi.org/10.1038/s41598-019-53049-w">https://doi.org/10.1038/s41598-019-53049-w</a>
  chicago: Maes, Margaret E, J. A. Grosser, R. L. Fehrman, C. L. Schlamp, and R. W.
    Nickells. “Completion of BAX Recruitment Correlates with Mitochondrial Fission
    during Apoptosis.” <i>Scientific Reports</i>. Springer Nature, 2019. <a href="https://doi.org/10.1038/s41598-019-53049-w">https://doi.org/10.1038/s41598-019-53049-w</a>.
  ieee: M. E. Maes, J. A. Grosser, R. L. Fehrman, C. L. Schlamp, and R. W. Nickells,
    “Completion of BAX recruitment correlates with mitochondrial fission during apoptosis,”
    <i>Scientific Reports</i>, vol. 9. Springer Nature, 2019.
  ista: Maes ME, Grosser JA, Fehrman RL, Schlamp CL, Nickells RW. 2019. Completion
    of BAX recruitment correlates with mitochondrial fission during apoptosis. Scientific
    Reports. 9, 16565.
  mla: Maes, Margaret E., et al. “Completion of BAX Recruitment Correlates with Mitochondrial
    Fission during Apoptosis.” <i>Scientific Reports</i>, vol. 9, 16565, Springer
    Nature, 2019, doi:<a href="https://doi.org/10.1038/s41598-019-53049-w">10.1038/s41598-019-53049-w</a>.
  short: M.E. Maes, J.A. Grosser, R.L. Fehrman, C.L. Schlamp, R.W. Nickells, Scientific
    Reports 9 (2019).
date_created: 2019-11-25T07:45:17Z
date_published: 2019-11-12T00:00:00Z
date_updated: 2023-08-30T07:26:54Z
day: '12'
ddc:
- '570'
department:
- _id: SaSi
doi: 10.1038/s41598-019-53049-w
external_id:
  isi:
  - '000495857600019'
  pmid:
  - '31719602'
file:
- access_level: open_access
  checksum: 9ab397ed9c1c454b34bffb8cc863d734
  content_type: application/pdf
  creator: dernst
  date_created: 2019-11-25T07:49:52Z
  date_updated: 2020-07-14T12:47:49Z
  file_id: '7096'
  file_name: 2019_ScientificReports_Maes.pdf
  file_size: 6467393
  relation: main_file
file_date_updated: 2020-07-14T12:47:49Z
fulldoi: https://doi.org/10.1038/s41598-019-53049-w
has_accepted_license: '1'
intvolume: '         9'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Scientific Reports
publication_identifier:
  eissn:
  - 2045-2322
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Completion of BAX recruitment correlates with mitochondrial fission during
  apoptosis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2019'
...