---
_id: '7105'
abstract:
- lang: eng
  text: Cell migration is hypothesized to involve a cycle of behaviours beginning
    with leading edge extension. However, recent evidence suggests that the leading
    edge may be dispensable for migration, raising the question of what actually controls
    cell directionality. Here, we exploit the embryonic migration of Drosophila macrophages
    to bridge the different temporal scales of the behaviours controlling motility.
    This approach reveals that edge fluctuations during random motility are not persistent
    and are weakly correlated with motion. In contrast, flow of the actin network
    behind the leading edge is highly persistent. Quantification of actin flow structure
    during migration reveals a stable organization and asymmetry in the cell-wide
    flowfield that strongly correlates with cell directionality. This organization
    is regulated by a gradient of actin network compression and destruction, which
    is controlled by myosin contraction and cofilin-mediated disassembly. It is this
    stable actin-flow polarity, which integrates rapid fluctuations of the leading
    edge, that controls inherent cellular persistence.
article_processing_charge: No
article_type: original
author:
- first_name: Lawrence
  full_name: Yolland, Lawrence
  last_name: Yolland
- first_name: Mubarik
  full_name: Burki, Mubarik
  last_name: Burki
- first_name: Stefania
  full_name: Marcotti, Stefania
  last_name: Marcotti
- first_name: Andrei
  full_name: Luchici, Andrei
  last_name: Luchici
- first_name: Fiona N.
  full_name: Kenny, Fiona N.
  last_name: Kenny
- first_name: John Robert
  full_name: Davis, John Robert
  last_name: Davis
- first_name: Eduardo
  full_name: Serna-Morales, Eduardo
  last_name: Serna-Morales
- first_name: Jan
  full_name: Müller, Jan
  id: AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D
  last_name: Müller
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Andrew
  full_name: Davidson, Andrew
  last_name: Davidson
- first_name: Will
  full_name: Wood, Will
  last_name: Wood
- first_name: Linus J.
  full_name: Schumacher, Linus J.
  last_name: Schumacher
- first_name: Robert G.
  full_name: Endres, Robert G.
  last_name: Endres
- first_name: Mark
  full_name: Miodownik, Mark
  last_name: Miodownik
- first_name: Brian M.
  full_name: Stramer, Brian M.
  last_name: Stramer
citation:
  ama: Yolland L, Burki M, Marcotti S, et al. Persistent and polarized global actin
    flow is essential for directionality during cell migration. <i>Nature Cell Biology</i>.
    2019;21(11):1370-1381. doi:<a href="https://doi.org/10.1038/s41556-019-0411-5">10.1038/s41556-019-0411-5</a>
  apa: Yolland, L., Burki, M., Marcotti, S., Luchici, A., Kenny, F. N., Davis, J.
    R., … Stramer, B. M. (2019). Persistent and polarized global actin flow is essential
    for directionality during cell migration. <i>Nature Cell Biology</i>. Springer
    Nature. <a href="https://doi.org/10.1038/s41556-019-0411-5">https://doi.org/10.1038/s41556-019-0411-5</a>
  chicago: Yolland, Lawrence, Mubarik Burki, Stefania Marcotti, Andrei Luchici, Fiona
    N. Kenny, John Robert Davis, Eduardo Serna-Morales, et al. “Persistent and Polarized
    Global Actin Flow Is Essential for Directionality during Cell Migration.” <i>Nature
    Cell Biology</i>. Springer Nature, 2019. <a href="https://doi.org/10.1038/s41556-019-0411-5">https://doi.org/10.1038/s41556-019-0411-5</a>.
  ieee: L. Yolland <i>et al.</i>, “Persistent and polarized global actin flow is essential
    for directionality during cell migration,” <i>Nature Cell Biology</i>, vol. 21,
    no. 11. Springer Nature, pp. 1370–1381, 2019.
  ista: Yolland L, Burki M, Marcotti S, Luchici A, Kenny FN, Davis JR, Serna-Morales
    E, Müller J, Sixt MK, Davidson A, Wood W, Schumacher LJ, Endres RG, Miodownik
    M, Stramer BM. 2019. Persistent and polarized global actin flow is essential for
    directionality during cell migration. Nature Cell Biology. 21(11), 1370–1381.
  mla: Yolland, Lawrence, et al. “Persistent and Polarized Global Actin Flow Is Essential
    for Directionality during Cell Migration.” <i>Nature Cell Biology</i>, vol. 21,
    no. 11, Springer Nature, 2019, pp. 1370–81, doi:<a href="https://doi.org/10.1038/s41556-019-0411-5">10.1038/s41556-019-0411-5</a>.
  short: L. Yolland, M. Burki, S. Marcotti, A. Luchici, F.N. Kenny, J.R. Davis, E.
    Serna-Morales, J. Müller, M.K. Sixt, A. Davidson, W. Wood, L.J. Schumacher, R.G.
    Endres, M. Miodownik, B.M. Stramer, Nature Cell Biology 21 (2019) 1370–1381.
date_created: 2019-11-25T08:55:00Z
date_published: 2019-11-01T00:00:00Z
date_updated: 2023-09-06T11:08:52Z
day: '01'
department:
- _id: MiSi
doi: 10.1038/s41556-019-0411-5
external_id:
  isi:
  - '000495888300009'
  pmid:
  - '31685997'
intvolume: '        21'
isi: 1
issue: '11'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025891
month: '11'
oa: 1
oa_version: Submitted Version
page: 1370-1381
pmid: 1
publication: Nature Cell Biology
publication_identifier:
  eissn:
  - 1476-4679
  issn:
  - 1465-7392
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Persistent and polarized global actin flow is essential for directionality
  during cell migration
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 21
year: '2019'
...