[{"month":"09","publication_identifier":{"issn":[]},"language":[{}],"oa":1,"external_id":{"pmid":[]},"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797705"}],"quality_controlled":"1","publist_id":"6967","creator":{"login":"apreinsp","id":"4435EBFC-F248-11E8-B48F-1D18A9856A87"},"author":[{"last_name":"Brailoiu","first_name":"Gabriela"},{"id":"37A40D7E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7370-5293","first_name":"Elena","last_name":"Deliu"},{"last_name":"Barr","first_name":"Jeffrey"},{"last_name":"Console Bram","first_name":"Linda"},{"first_name":"Alexandra","last_name":"Ciuciu"},{"first_name":"Mary","last_name":"Abood"},{"first_name":"Ellen","last_name":"Unterwald"},{"last_name":"Brǎiloiu","first_name":"Eugen"}],"date_created":"2018-12-11T11:48:05Z","dini_type":"doc-type:article","date_updated":"2021-01-12T08:12:00Z","volume":178,"acknowledgement":"This work was supported by the National Institutes of Health grants DA035926 (to MEA), and P30DA013429 (to EMU).","pmid":1,"publication_status":"published","department":[{"_id":"GaNo","tree":[{"_id":"ResearchGroups"},{"_id":"IST"}]}],"day":"01","uri_base":"https://research-explorer.ista.ac.at","article_processing_charge":"No","scopus_import":1,"dc":{"identifier":["https://research-explorer.ista.ac.at/record/714"],"type":["info:eu-repo/semantics/article","doc-type:article","text","http://purl.org/coar/resource_type/c_6501"],"description":["Background HIV-1 infection and drug abuse are frequently co-morbid and their association greatly increases the severity of HIV-1-induced neuropathology. While nucleus accumbens (NAcc) function is severely perturbed by drugs of abuse, little is known about how HIV-1 infection affects NAcc. Methods We used calcium and voltage imaging to investigate the effect of HIV-1 trans-activator of transcription (Tat) on rat NAcc. Based on previous neuronal studies, we hypothesized that Tat modulates intracellular Ca2+ homeostasis of NAcc neurons. Results We provide evidence that Tat triggers a Ca2+ signaling cascade in NAcc medium spiny neurons (MSN) expressing D1-like dopamine receptors leading to neuronal depolarization. Firstly, Tat induced inositol 1,4,5-trisphsophate (IP3) receptor-mediated Ca2+ release from endoplasmic reticulum, followed by Ca2+ and Na+ influx via transient receptor potential canonical channels. The influx of cations depolarizes the membrane promoting additional Ca2+ entry through voltage-gated P/Q-type Ca2+ channels and opening of tetrodotoxin-sensitive Na+ channels. By activating this mechanism, Tat elicits a feed-forward depolarization increasing the excitability of D1-phosphatidylinositol-linked NAcc MSN. We previously found that cocaine targets NAcc neurons directly (independent of the inhibition of dopamine transporter) only when IP3-generating mechanisms are concomitantly initiated. When tested here, cocaine produced a dose-dependent potentiation of the effect of Tat on cytosolic Ca2+. Conclusion We describe for the first time a HIV-1 Tat-triggered Ca2+ signaling in MSN of NAcc involving TRPC and depolarization and a potentiation of the effect of Tat by cocaine, which may be relevant for the reward axis in cocaine-abusing HIV-1-positive patients."],"creator":["Brailoiu, Gabriela","Deliu, Elena","Barr, Jeffrey","Console Bram, Linda","Ciuciu, Alexandra","Abood, Mary","Unterwald, Ellen","Brǎiloiu, Eugen"],"source":["Brailoiu G, Deliu E, Barr J, et al. HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens. Drug and Alcohol Dependence. 2017;178:7-14. doi:10.1016/j.drugalcdep.2017.04.015"],"rights":["info:eu-repo/semantics/openAccess"],"publisher":["Elsevier"],"relation":["info:eu-repo/semantics/altIdentifier/doi/10.1016/j.drugalcdep.2017.04.015","info:eu-repo/semantics/altIdentifier/issn/03768716","info:eu-repo/semantics/altIdentifier/pmid/28623807"],"title":["HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens"],"date":["2017"],"language":["eng"]},"date_published":"2017-09-01T00:00:00Z","publication":"Drug and Alcohol Dependence","citation":{"ista":"Brailoiu G, Deliu E, Barr J, Console Bram L, Ciuciu A, Abood M, Unterwald E, Brǎiloiu E. 2017. HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens. Drug and Alcohol Dependence. 178, 7–14.","apa":"Brailoiu, G., Deliu, E., Barr, J., Console Bram, L., Ciuciu, A., Abood, M., … Brǎiloiu, E. (2017). HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens. Drug and Alcohol Dependence. Elsevier. https://doi.org/10.1016/j.drugalcdep.2017.04.015","ieee":"G. Brailoiu et al., “HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens,” Drug and Alcohol Dependence, vol. 178. Elsevier, pp. 7–14, 2017.","mla":"Brailoiu, Gabriela, et al. “HIV Tat Excites D1 Receptor-like Expressing Neurons from Rat Nucleus Accumbens.” Drug and Alcohol Dependence, vol. 178, Elsevier, 2017, pp. 7–14, doi:10.1016/j.drugalcdep.2017.04.015.","short":"G. Brailoiu, E. Deliu, J. Barr, L. Console Bram, A. Ciuciu, M. Abood, E. Unterwald, E. Brǎiloiu, Drug and Alcohol Dependence 178 (2017) 7–14.","chicago":"Brailoiu, Gabriela, Elena Deliu, Jeffrey Barr, Linda Console Bram, Alexandra Ciuciu, Mary Abood, Ellen Unterwald, and Eugen Brǎiloiu. “HIV Tat Excites D1 Receptor-like Expressing Neurons from Rat Nucleus Accumbens.” Drug and Alcohol Dependence. Elsevier, 2017. https://doi.org/10.1016/j.drugalcdep.2017.04.015."},"article_type":"original","page":"7 - 14","abstract":[{"lang":"eng"}],"type":"journal_article","oa_version":"Submitted Version","_id":"714","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","intvolume":" 178"}]