---
_id: '7197'
abstract:
- lang: eng
  text: During bacterial cell division, the tubulin-homolog FtsZ forms a ring-like
    structure at the center of the cell. This Z-ring not only organizes the division
    machinery, but treadmilling of FtsZ filaments was also found to play a key role
    in distributing proteins at the division site. What regulates the architecture,
    dynamics and stability of the Z-ring is currently unknown, but FtsZ-associated
    proteins are known to play an important role. Here, using an in vitro reconstitution
    approach, we studied how the well-conserved protein ZapA affects FtsZ treadmilling
    and filament organization into large-scale patterns. Using high-resolution fluorescence
    microscopy and quantitative image analysis, we found that ZapA cooperatively increases
    the spatial order of the filament network, but binds only transiently to FtsZ
    filaments and has no effect on filament length and treadmilling velocity. Together,
    our data provides a model for how FtsZ-associated proteins can increase the precision
    and stability of the bacterial cell division machinery in a switch-like manner.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
article_number: '5744'
article_processing_charge: No
article_type: original
author:
- first_name: Paulo R
  full_name: Dos Santos Caldas, Paulo R
  id: 38FCDB4C-F248-11E8-B48F-1D18A9856A87
  last_name: Dos Santos Caldas
  orcid: 0000-0001-6730-4461
- first_name: Maria D
  full_name: Lopez Pelegrin, Maria D
  id: 319AA9CE-F248-11E8-B48F-1D18A9856A87
  last_name: Lopez Pelegrin
- first_name: Daniel J. G.
  full_name: Pearce, Daniel J. G.
  last_name: Pearce
- first_name: Nazmi B
  full_name: Budanur, Nazmi B
  id: 3EA1010E-F248-11E8-B48F-1D18A9856A87
  last_name: Budanur
  orcid: 0000-0003-0423-5010
- first_name: Jan
  full_name: Brugués, Jan
  last_name: Brugués
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
citation:
  ama: Dos Santos Caldas PR, Lopez Pelegrin MD, Pearce DJG, Budanur NB, Brugués J,
    Loose M. Cooperative ordering of treadmilling filaments in cytoskeletal networks
    of FtsZ and its crosslinker ZapA. <i>Nature Communications</i>. 2019;10. doi:<a
    href="https://doi.org/10.1038/s41467-019-13702-4">10.1038/s41467-019-13702-4</a>
  apa: Dos Santos Caldas, P. R., Lopez Pelegrin, M. D., Pearce, D. J. G., Budanur,
    N. B., Brugués, J., &#38; Loose, M. (2019). Cooperative ordering of treadmilling
    filaments in cytoskeletal networks of FtsZ and its crosslinker ZapA. <i>Nature
    Communications</i>. Springer Nature. <a href="https://doi.org/10.1038/s41467-019-13702-4">https://doi.org/10.1038/s41467-019-13702-4</a>
  chicago: Dos Santos Caldas, Paulo R, Maria D Lopez Pelegrin, Daniel J. G. Pearce,
    Nazmi B Budanur, Jan Brugués, and Martin Loose. “Cooperative Ordering of Treadmilling
    Filaments in Cytoskeletal Networks of FtsZ and Its Crosslinker ZapA.” <i>Nature
    Communications</i>. Springer Nature, 2019. <a href="https://doi.org/10.1038/s41467-019-13702-4">https://doi.org/10.1038/s41467-019-13702-4</a>.
  ieee: P. R. Dos Santos Caldas, M. D. Lopez Pelegrin, D. J. G. Pearce, N. B. Budanur,
    J. Brugués, and M. Loose, “Cooperative ordering of treadmilling filaments in cytoskeletal
    networks of FtsZ and its crosslinker ZapA,” <i>Nature Communications</i>, vol.
    10. Springer Nature, 2019.
  ista: Dos Santos Caldas PR, Lopez Pelegrin MD, Pearce DJG, Budanur NB, Brugués J,
    Loose M. 2019. Cooperative ordering of treadmilling filaments in cytoskeletal
    networks of FtsZ and its crosslinker ZapA. Nature Communications. 10, 5744.
  mla: Dos Santos Caldas, Paulo R., et al. “Cooperative Ordering of Treadmilling Filaments
    in Cytoskeletal Networks of FtsZ and Its Crosslinker ZapA.” <i>Nature Communications</i>,
    vol. 10, 5744, Springer Nature, 2019, doi:<a href="https://doi.org/10.1038/s41467-019-13702-4">10.1038/s41467-019-13702-4</a>.
  short: P.R. Dos Santos Caldas, M.D. Lopez Pelegrin, D.J.G. Pearce, N.B. Budanur,
    J. Brugués, M. Loose, Nature Communications 10 (2019).
corr_author: '1'
date_created: 2019-12-20T12:22:57Z
date_published: 2019-12-17T00:00:00Z
date_updated: 2026-04-08T07:26:30Z
day: '17'
ddc:
- '570'
department:
- _id: MaLo
- _id: BjHo
doi: 10.1038/s41467-019-13702-4
ec_funded: 1
external_id:
  isi:
  - '000503009300001'
file:
- access_level: open_access
  checksum: a1b44b427ba341383197790d0e8789fa
  content_type: application/pdf
  creator: dernst
  date_created: 2019-12-23T07:34:56Z
  date_updated: 2020-07-14T12:47:53Z
  file_id: '7208'
  file_name: 2019_NatureComm_Caldas.pdf
  file_size: 8488733
  relation: main_file
file_date_updated: 2020-07-14T12:47:53Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
project:
- _id: 2595697A-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '679239'
  name: Self-Organization of the Bacterial Cell
- _id: 260D98C8-B435-11E9-9278-68D0E5697425
  name: Reconstitution of Bacterial Cell Division Using Purified Components
publication: Nature Communications
publication_identifier:
  issn:
  - 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  record:
  - id: '8358'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Cooperative ordering of treadmilling filaments in cytoskeletal networks of
  FtsZ and its crosslinker ZapA
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 10
year: '2019'
...