article published yes Daniel Aigner author Stefan Alexander Freunberger author A8CA28E6-CE23-11E9-AD2D-EC27E66974250000-0003-2902-5319 Martin Wilkening author Robert Saf author Sergey M. Borisov author Ingo Klimant author Photoinduced electron transfer (PET), which causes pH-dependent quenching of fluorescent dyes, is more effectively introduced by phenolic groups than by amino groups which have been much more commonly used so far. That is demonstrated by fluorescence measurements involving several classes of fluorophores. Electrochemical measurements show that PET in several amino-modified dyes is thermodynamically favorable, even though it was not experimentally found, underlining the importance of kinetic aspects to the process. Consequently, the attachment of phenolic groups allows for fast and simple preparation of a wide selection of fluorescent pH-probes with tailor-made spectral properties, sensitive ranges, and individual advantages, so that a large number of applications can be realized. Fluorophores carrying phenolic groups may also be used for sensing analytes other than pH or molecular switching and signaling. ACS2014 eng 0003-2700 1520-688210.1021/ac502513g 86189293-9300 yes D. Aigner, S. A. Freunberger, M. Wilkening, R. Saf, S. M. Borisov, and I. Klimant, “Enhancing photoinduced electron transfer efficiency of fluorescent pH-probes with halogenated phenols,” <i>Analytical Chemistry</i>, vol. 86, no. 18. ACS, pp. 9293–9300, 2014. D. Aigner, S.A. Freunberger, M. Wilkening, R. Saf, S.M. Borisov, I. Klimant, Analytical Chemistry 86 (2014) 9293–9300. Aigner D, Freunberger SA, Wilkening M, Saf R, Borisov SM, Klimant I. 2014. Enhancing photoinduced electron transfer efficiency of fluorescent pH-probes with halogenated phenols. Analytical Chemistry. 86(18), 9293–9300. Aigner, D., Freunberger, S. A., Wilkening, M., Saf, R., Borisov, S. M., &#38; Klimant, I. (2014). Enhancing photoinduced electron transfer efficiency of fluorescent pH-probes with halogenated phenols. <i>Analytical Chemistry</i>. ACS. <a href="https://doi.org/10.1021/ac502513g">https://doi.org/10.1021/ac502513g</a> Aigner, Daniel, et al. “Enhancing Photoinduced Electron Transfer Efficiency of Fluorescent PH-Probes with Halogenated Phenols.” <i>Analytical Chemistry</i>, vol. 86, no. 18, ACS, 2014, pp. 9293–300, doi:<a href="https://doi.org/10.1021/ac502513g">10.1021/ac502513g</a>. Aigner, Daniel, Stefan Alexander Freunberger, Martin Wilkening, Robert Saf, Sergey M. Borisov, and Ingo Klimant. “Enhancing Photoinduced Electron Transfer Efficiency of Fluorescent PH-Probes with Halogenated Phenols.” <i>Analytical Chemistry</i>. ACS, 2014. <a href="https://doi.org/10.1021/ac502513g">https://doi.org/10.1021/ac502513g</a>. Aigner D, Freunberger SA, Wilkening M, Saf R, Borisov SM, Klimant I. Enhancing photoinduced electron transfer efficiency of fluorescent pH-probes with halogenated phenols. <i>Analytical Chemistry</i>. 2014;86(18):9293-9300. doi:<a href="https://doi.org/10.1021/ac502513g">10.1021/ac502513g</a> 73002020-01-15T12:17:17Z2021-01-12T08:12:53Z