---
_id: '7358'
abstract:
- lang: eng
  text: Telencephalic organoids generated from human pluripotent stem cells (hPSCs)
    are emerging as an effective system to study the distinct features of the developing
    human brain and the underlying causes of many neurological disorders. While progress
    in organoid technology has been steadily advancing, many challenges remain including
    rampant batch-to-batch and cell line-to-cell line variability and irreproducibility.
    Here, we demonstrate that a major contributor to successful cortical organoid
    production is the manner in which hPSCs are maintained prior to differentiation.
    Optimal results were achieved using fibroblast-feeder-supported hPSCs compared
    to feeder-independent cells, related to differences in their transcriptomic states.
    Feeder-supported hPSCs display elevated activation of diverse TGFβ superfamily
    signaling pathways and increased expression of genes associated with naïve pluripotency.
    We further identify combinations of TGFβ-related growth factors that are necessary
    and together sufficient to impart broad telencephalic organoid competency to feeder-free
    hPSCs and enable reproducible formation of brain structures suitable for disease
    modeling.
article_processing_charge: No
author:
- first_name: Momoko
  full_name: Watanabe, Momoko
  last_name: Watanabe
- first_name: Jillian R.
  full_name: Haney, Jillian R.
  last_name: Haney
- first_name: Neda
  full_name: Vishlaghi, Neda
  last_name: Vishlaghi
- first_name: Felix
  full_name: Turcios, Felix
  last_name: Turcios
- first_name: Jessie E.
  full_name: Buth, Jessie E.
  last_name: Buth
- first_name: Wen
  full_name: Gu, Wen
  last_name: Gu
- first_name: Amanda J.
  full_name: Collier, Amanda J.
  last_name: Collier
- first_name: Osvaldo
  full_name: Miranda, Osvaldo
  id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
  last_name: Miranda
  orcid: 0000-0001-6618-6889
- first_name: Di
  full_name: Chen, Di
  last_name: Chen
- first_name: Shan
  full_name: Sabri, Shan
  last_name: Sabri
- first_name: Amander T.
  full_name: Clark, Amander T.
  last_name: Clark
- first_name: Kathrin
  full_name: Plath, Kathrin
  last_name: Plath
- first_name: Heather R.
  full_name: Christofk, Heather R.
  last_name: Christofk
- first_name: Michael J.
  full_name: Gandal, Michael J.
  last_name: Gandal
- first_name: Bennett G.
  full_name: Novitch, Bennett G.
  last_name: Novitch
citation:
  ama: Watanabe M, Haney JR, Vishlaghi N, et al. TGFβ superfamily signaling regulates
    the state of human stem cell pluripotency and competency to create telencephalic
    organoids. <i>bioRxiv</i>. 2019. doi:<a href="https://doi.org/10.1101/2019.12.13.875773">10.1101/2019.12.13.875773</a>
  apa: Watanabe, M., Haney, J. R., Vishlaghi, N., Turcios, F., Buth, J. E., Gu, W.,
    … Novitch, B. G. (2019). TGFβ superfamily signaling regulates the state of human
    stem cell pluripotency and competency to create telencephalic organoids. <i>bioRxiv</i>.
    Cold Spring Harbor Laboratory. <a href="https://doi.org/10.1101/2019.12.13.875773">https://doi.org/10.1101/2019.12.13.875773</a>
  chicago: Watanabe, Momoko, Jillian R. Haney, Neda Vishlaghi, Felix Turcios, Jessie
    E. Buth, Wen Gu, Amanda J. Collier, et al. “TGFβ Superfamily Signaling Regulates
    the State of Human Stem Cell Pluripotency and Competency to Create Telencephalic
    Organoids.” <i>BioRxiv</i>. Cold Spring Harbor Laboratory, 2019. <a href="https://doi.org/10.1101/2019.12.13.875773">https://doi.org/10.1101/2019.12.13.875773</a>.
  ieee: M. Watanabe <i>et al.</i>, “TGFβ superfamily signaling regulates the state
    of human stem cell pluripotency and competency to create telencephalic organoids,”
    <i>bioRxiv</i>. Cold Spring Harbor Laboratory, 2019.
  ista: Watanabe M, Haney JR, Vishlaghi N, Turcios F, Buth JE, Gu W, Collier AJ, Miranda
    O, Chen D, Sabri S, Clark AT, Plath K, Christofk HR, Gandal MJ, Novitch BG. 2019.
    TGFβ superfamily signaling regulates the state of human stem cell pluripotency
    and competency to create telencephalic organoids. bioRxiv, <a href="https://doi.org/10.1101/2019.12.13.875773">10.1101/2019.12.13.875773</a>.
  mla: Watanabe, Momoko, et al. “TGFβ Superfamily Signaling Regulates the State of
    Human Stem Cell Pluripotency and Competency to Create Telencephalic Organoids.”
    <i>BioRxiv</i>, Cold Spring Harbor Laboratory, 2019, doi:<a href="https://doi.org/10.1101/2019.12.13.875773">10.1101/2019.12.13.875773</a>.
  short: M. Watanabe, J.R. Haney, N. Vishlaghi, F. Turcios, J.E. Buth, W. Gu, A.J.
    Collier, O. Miranda, D. Chen, S. Sabri, A.T. Clark, K. Plath, H.R. Christofk,
    M.J. Gandal, B.G. Novitch, BioRxiv (2019).
date_created: 2020-01-23T09:53:40Z
date_published: 2019-12-13T00:00:00Z
date_updated: 2022-06-17T08:03:32Z
day: '13'
doi: 10.1101/2019.12.13.875773
extern: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/2019.12.13.875773
month: '12'
oa: 1
oa_version: Preprint
page: '75'
publication: bioRxiv
publication_status: published
publisher: Cold Spring Harbor Laboratory
status: public
title: TGFβ superfamily signaling regulates the state of human stem cell pluripotency
  and competency to create telencephalic organoids
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2019'
...