---
res:
  bibo_abstract:
  - 'Transporters of the solute carrier 6 (SLC6) family translocate their cognate
    substrate together with Na+ and Cl−. Detailed kinetic models exist for the transporters
    of GABA (GAT1/SLC6A1) and the monoamines dopamine (DAT/SLC6A3) and serotonin (SERT/SLC6A4).
    Here, we posited that the transport cycle of individual SLC6 transporters reflects
    the physiological requirements they operate under. We tested this hypothesis by
    analyzing the transport cycle of glycine transporter 1 (GlyT1/SLC6A9) and glycine
    transporter 2 (GlyT2/SLC6A5). GlyT2 is the only SLC6 family member known to translocate
    glycine, Na+, and Cl− in a 1:3:1 stoichiometry. We analyzed partial reactions
    in real time by electrophysiological recordings. Contrary to monoamine transporters,
    both GlyTs were found to have a high transport capacity driven by rapid return
    of the empty transporter after release of Cl− on the intracellular side. Rapid
    cycling of both GlyTs was further supported by highly cooperative binding of cosubstrate
    ions and substrate such that their forward transport mode was maintained even
    under conditions of elevated intracellular Na+ or Cl−. The most important differences
    in the transport cycle of GlyT1 and GlyT2 arose from the kinetics of charge movement
    and the resulting voltage-dependent rate-limiting reactions: the kinetics of GlyT1
    were governed by transition of the substrate-bound transporter from outward- to
    inward-facing conformations, whereas the kinetics of GlyT2 were governed by Na+
    binding (or a related conformational change). Kinetic modeling showed that the
    kinetics of GlyT1 are ideally suited for supplying the extracellular glycine levels
    required for NMDA receptor activation.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Fatma Asli
      foaf_name: Erdem, Fatma Asli
      foaf_surname: Erdem
  - foaf_Person:
      foaf_givenName: Marija
      foaf_name: Ilic, Marija
      foaf_surname: Ilic
  - foaf_Person:
      foaf_givenName: Peter
      foaf_name: Koppensteiner, Peter
      foaf_surname: Koppensteiner
      foaf_workInfoHomepage: http://www.librecat.org/personId=3B8B25A8-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-3509-1948
  - foaf_Person:
      foaf_givenName: Jakub
      foaf_name: Gołacki, Jakub
      foaf_surname: Gołacki
  - foaf_Person:
      foaf_givenName: Gert
      foaf_name: Lubec, Gert
      foaf_surname: Lubec
  - foaf_Person:
      foaf_givenName: Michael
      foaf_name: Freissmuth, Michael
      foaf_surname: Freissmuth
  - foaf_Person:
      foaf_givenName: Walter
      foaf_name: Sandtner, Walter
      foaf_surname: Sandtner
  bibo_doi: 10.1085/jgp.201912318
  bibo_issue: '8'
  bibo_volume: 151
  dct_date: 2019^xs_gYear
  dct_identifier:
  - UT:000478792500008
  dct_isPartOf:
  - http://id.crossref.org/issn/0022-1295
  - http://id.crossref.org/issn/1540-7748
  dct_language: eng
  dct_publisher: Rockefeller University Press@
  dct_title: A comparison of the transport kinetics of glycine transporter 1 and glycine
    transporter 2@
  fabio_hasPubmedId: '31270129'
...