article published yes Ewa Mazur author Michelle C Gallei author 35A03822-F248-11E8-B48F-1D18A9856A870000-0003-1286-7368 Maciek Adamowski author 45F536D2-F248-11E8-B48F-1D18A9856A870000-0001-6463-5257 Huibin Han author 31435098-F248-11E8-B48F-1D18A9856A87 Hélène S. Robert author Jiří Friml author 4159519E-F248-11E8-B48F-1D18A9856A870000-0002-8302-7596 JiFr department Tracing Evolution of Auxin Transport and Polarity in Plants project The flexible development of plants is characterized by a high capacity for post-embryonic organ formation and tissue regeneration, processes, which require tightly regulated intercellular communication and coordinated tissue (re-)polarization. The phytohormone auxin, the main driver for these processes, is able to establish polarized auxin transport channels, which are characterized by the expression and polar, subcellular localization of the PIN1 auxin transport proteins. These channels are demarcating the position of future vascular strands necessary for organ formation and tissue regeneration. Major progress has been made in the last years to understand how PINs can change their polarity in different contexts and thus guide auxin flow through the plant. However, it still remains elusive how auxin mediates the establishment of auxin conducting channels and the formation of vascular tissue and which cellular processes are involved. By the means of sophisticated regeneration experiments combined with local auxin applications in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics, (ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact actin cytoskeleton required for post-endocytic trafficking are indispensable for auxin channel formation, de novo vascular formation and vascular regeneration after wounding. These observations provide novel insights into cellular mechanism of coordinated tissue polarization during auxin canalization. https://research-explorer.ista.ac.at/download/7465/7471/2020_PlantScience_Mazur.pdf application/pdfno https://creativecommons.org/licenses/by/4.0/ Elsevier2020 eng 0168-9452 1873-2259 32081263 00052060980000910.1016/j.plantsci.2020.110414 2934 https://research-explorer.ista.ac.at/record/11626 Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. 2020. Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. Plant Science. 293(4), 110414. Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. <i>Plant Science</i>. 2020;293(4). doi:<a href="https://doi.org/10.1016/j.plantsci.2020.110414">10.1016/j.plantsci.2020.110414</a> Mazur, Ewa, et al. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” <i>Plant Science</i>, vol. 293, no. 4, 110414, Elsevier, 2020, doi:<a href="https://doi.org/10.1016/j.plantsci.2020.110414">10.1016/j.plantsci.2020.110414</a>. E. Mazur, M. C. Gallei, M. Adamowski, H. Han, H. S. Robert, and J. Friml, “Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis,” <i>Plant Science</i>, vol. 293, no. 4. Elsevier, 2020. Mazur, Ewa, Michelle C Gallei, Maciek Adamowski, Huibin Han, Hélène S. Robert, and Jiří Friml. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” <i>Plant Science</i>. Elsevier, 2020. <a href="https://doi.org/10.1016/j.plantsci.2020.110414">https://doi.org/10.1016/j.plantsci.2020.110414</a>. E. Mazur, M.C. Gallei, M. Adamowski, H. Han, H.S. Robert, J. Friml, Plant Science 293 (2020). Mazur, E., Gallei, M. C., Adamowski, M., Han, H., Robert, H. S., &#38; Friml, J. (2020). Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. <i>Plant Science</i>. Elsevier. <a href="https://doi.org/10.1016/j.plantsci.2020.110414">https://doi.org/10.1016/j.plantsci.2020.110414</a> 74652020-02-09T23:00:50Z2026-04-07T14:18:57Z