article published yes Chong Chen author 3DFD581A-F248-11E8-B48F-1D18A9856A87 Rachel Satterfield author Samuel Young author Peter M Jonas author 353C1B58-F248-11E8-B48F-1D18A9856A870000-0001-5001-4804 PeJo department Mechanisms of transmitter release at GABAergic synapses project Biophysics and circuit function of a giant cortical glutamatergic synapse project Synaptotagmin 7 (Syt7) is thought to be a Ca2+ sensor that mediates asynchronous transmitter release and facilitation at synapses. However, Syt7 is strongly expressed in fast-spiking, parvalbumin-expressing GABAergic interneurons, and the output synapses of these neurons produce only minimal asynchronous release and show depression rather than facilitation. To resolve this apparent contradiction, we examined the effects of genetic elimination of Syt7 on synaptic transmission at the GABAergic basket cell (BC)-Purkinje cell (PC) synapse in cerebellum. Our results indicate that at the BC-PC synapse, Syt7 contributes to asynchronous release, pool replenishment, and facilitation. In combination, these three effects ensure efficient transmitter release during high-frequency activity and guarantee frequency independence of inhibition. Our results identify a distinct function of Syt7: ensuring the efficiency of high-frequency inhibitory synaptic transmission https://research-explorer.ista.ac.at/download/749/4737/IST-2017-874-v1+1_PIIS2211124717316029.pdf application/pdfno https://creativecommons.org/licenses/by/4.0/ Cell Press2017 eng 2211-1247 00041621670000710.1016/j.celrep.2017.10.122 2182082 - 2089 https://research-explorer.ista.ac.at/record/324 C. Chen, R. Satterfield, S. Young, and P. M. Jonas, “Triple function of Synaptotagmin 7 ensures efficiency of high-frequency transmission at central GABAergic synapses,” <i>Cell Reports</i>, vol. 21, no. 8. Cell Press, pp. 2082–2089, 2017. Chen, Chong, Rachel Satterfield, Samuel Young, and Peter M Jonas. “Triple Function of Synaptotagmin 7 Ensures Efficiency of High-Frequency Transmission at Central GABAergic Synapses.” <i>Cell Reports</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.celrep.2017.10.122">https://doi.org/10.1016/j.celrep.2017.10.122</a>. Chen, C., Satterfield, R., Young, S., &#38; Jonas, P. M. (2017). Triple function of Synaptotagmin 7 ensures efficiency of high-frequency transmission at central GABAergic synapses. <i>Cell Reports</i>. Cell Press. <a href="https://doi.org/10.1016/j.celrep.2017.10.122">https://doi.org/10.1016/j.celrep.2017.10.122</a> C. Chen, R. Satterfield, S. Young, P.M. Jonas, Cell Reports 21 (2017) 2082–2089. Chen C, Satterfield R, Young S, Jonas PM. 2017. Triple function of Synaptotagmin 7 ensures efficiency of high-frequency transmission at central GABAergic synapses. Cell Reports. 21(8), 2082–2089. Chen C, Satterfield R, Young S, Jonas PM. Triple function of Synaptotagmin 7 ensures efficiency of high-frequency transmission at central GABAergic synapses. <i>Cell Reports</i>. 2017;21(8):2082-2089. doi:<a href="https://doi.org/10.1016/j.celrep.2017.10.122">10.1016/j.celrep.2017.10.122</a> Chen, Chong, et al. “Triple Function of Synaptotagmin 7 Ensures Efficiency of High-Frequency Transmission at Central GABAergic Synapses.” <i>Cell Reports</i>, vol. 21, no. 8, Cell Press, 2017, pp. 2082–89, doi:<a href="https://doi.org/10.1016/j.celrep.2017.10.122">10.1016/j.celrep.2017.10.122</a>. 7492018-12-11T11:48:18Z2026-04-08T14:09:28Z