---
_id: '7490'
abstract:
- lang: eng
  text: In plants, clathrin mediated endocytosis (CME) represents the major route
    for cargo internalisation from the cell surface. It has been assumed to operate
    in an evolutionary conserved manner as in yeast and animals. Here we report characterisation
    of ultrastructure, dynamics and mechanisms of plant CME as allowed by our advancement
    in electron microscopy and quantitative live imaging techniques. Arabidopsis CME
    appears to follow the constant curvature model and the bona fide CME population
    generates vesicles of a predominantly hexagonal-basket type; larger and with faster
    kinetics than in other models. Contrary to the existing paradigm, actin is dispensable
    for CME events at the plasma membrane but plays a unique role in collecting endocytic
    vesicles, sorting of internalised cargos and directional endosome movement that
    itself actively promote CME events. Internalized vesicles display a strongly delayed
    and sequential uncoating. These unique features highlight the independent evolution
    of the plant CME mechanism during the autonomous rise of multicellularity in eukaryotes.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
article_number: e52067
article_processing_charge: No
article_type: original
author:
- first_name: Madhumitha
  full_name: Narasimhan, Madhumitha
  id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
  last_name: Narasimhan
  orcid: 0000-0002-8600-0671
- first_name: Alexander J
  full_name: Johnson, Alexander J
  id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
  last_name: Johnson
  orcid: 0000-0002-2739-8843
- first_name: Roshan
  full_name: Prizak, Roshan
  id: 4456104E-F248-11E8-B48F-1D18A9856A87
  last_name: Prizak
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Barbara E
  full_name: Casillas Perez, Barbara E
  id: 351ED2AA-F248-11E8-B48F-1D18A9856A87
  last_name: Casillas Perez
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Narasimhan M, Johnson AJ, Prizak R, et al. Evolutionarily unique mechanistic
    framework of clathrin-mediated endocytosis in plants. <i>eLife</i>. 2020;9. doi:<a
    href="https://doi.org/10.7554/eLife.52067">10.7554/eLife.52067</a>
  apa: Narasimhan, M., Johnson, A. J., Prizak, R., Kaufmann, W., Tan, S., Casillas
    Perez, B. E., &#38; Friml, J. (2020). Evolutionarily unique mechanistic framework
    of clathrin-mediated endocytosis in plants. <i>ELife</i>. eLife Sciences Publications.
    <a href="https://doi.org/10.7554/eLife.52067">https://doi.org/10.7554/eLife.52067</a>
  chicago: Narasimhan, Madhumitha, Alexander J Johnson, Roshan Prizak, Walter Kaufmann,
    Shutang Tan, Barbara E Casillas Perez, and Jiří Friml. “Evolutionarily Unique
    Mechanistic Framework of Clathrin-Mediated Endocytosis in Plants.” <i>ELife</i>.
    eLife Sciences Publications, 2020. <a href="https://doi.org/10.7554/eLife.52067">https://doi.org/10.7554/eLife.52067</a>.
  ieee: M. Narasimhan <i>et al.</i>, “Evolutionarily unique mechanistic framework
    of clathrin-mediated endocytosis in plants,” <i>eLife</i>, vol. 9. eLife Sciences
    Publications, 2020.
  ista: Narasimhan M, Johnson AJ, Prizak R, Kaufmann W, Tan S, Casillas Perez BE,
    Friml J. 2020. Evolutionarily unique mechanistic framework of clathrin-mediated
    endocytosis in plants. eLife. 9, e52067.
  mla: Narasimhan, Madhumitha, et al. “Evolutionarily Unique Mechanistic Framework
    of Clathrin-Mediated Endocytosis in Plants.” <i>ELife</i>, vol. 9, e52067, eLife
    Sciences Publications, 2020, doi:<a href="https://doi.org/10.7554/eLife.52067">10.7554/eLife.52067</a>.
  short: M. Narasimhan, A.J. Johnson, R. Prizak, W. Kaufmann, S. Tan, B.E. Casillas
    Perez, J. Friml, ELife 9 (2020).
date_created: 2020-02-16T23:00:50Z
date_published: 2020-01-23T00:00:00Z
date_updated: 2025-04-14T07:45:03Z
day: '23'
ddc:
- '570'
- '580'
department:
- _id: JiFr
- _id: GaTk
- _id: EM-Fac
- _id: SyCr
doi: 10.7554/eLife.52067
ec_funded: 1
external_id:
  isi:
  - '000514104100001'
  pmid:
  - '31971511'
file:
- access_level: open_access
  checksum: 2052daa4be5019534f3a42f200a09f32
  content_type: application/pdf
  creator: dernst
  date_created: 2020-02-18T07:21:16Z
  date_updated: 2020-07-14T12:47:59Z
  file_id: '7494'
  file_name: 2020_eLife_Narasimhan.pdf
  file_size: 7247468
  relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: '         9'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
publication: eLife
publication_identifier:
  eissn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis
  in plants
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...