---
_id: '7548'
abstract:
- lang: eng
  text: Although the aggregation of the amyloid-β peptide (Aβ) into amyloid fibrils
    is a well-established hallmark of Alzheimer’s disease, the complex mechanisms
    linking this process to neurodegeneration are still incompletely understood. The
    nematode worm C. elegans is a valuable model organism through which to study these
    mechanisms because of its simple nervous system and its relatively short lifespan.
    Standard Aβ-based C. elegans models of Alzheimer’s disease are designed to study
    the toxic effects of the overexpression of Aβ in the muscle or nervous systems.
    However, the wide variety of effects associated with the tissue-level overexpression
    of Aβ makes it difficult to single out and study specific cellular mechanisms
    related to the onset of Alzheimer’s disease. Here, to better understand how to
    investigate the early events affecting neuronal signalling, we created a C. elegans
    model expressing Aβ42, the 42-residue form of Aβ, from a single-copy gene insertion
    in just one pair of glutamatergic sensory neurons, the BAG neurons. In behavioural
    assays, we found that the Aβ42-expressing animals displayed a subtle modulation
    of the response to CO2, compared to controls. Ca2+ imaging revealed that the BAG
    neurons in young Aβ42-expressing nematodes were activated more strongly than in
    control animals, and that neuronal activation remained intact until old age. Taken
    together, our results suggest that Aβ42-expression in this very subtle model of
    AD is sufficient to modulate the behavioural response but not strong enough to
    generate significant neurotoxicity, suggesting that slightly more aggressive perturbations
    will enable effectively studies of the links between the modulation of a physiological
    response and its associated neurotoxicity.
article_number: e0217746
article_processing_charge: No
article_type: original
author:
- first_name: Tessa
  full_name: Sinnige, Tessa
  last_name: Sinnige
- first_name: Prashanth
  full_name: Ciryam, Prashanth
  last_name: Ciryam
- first_name: Samuel
  full_name: Casford, Samuel
  last_name: Casford
- first_name: Christopher M.
  full_name: Dobson, Christopher M.
  last_name: Dobson
- first_name: Mario
  full_name: de Bono, Mario
  id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
  last_name: de Bono
  orcid: 0000-0001-8347-0443
- first_name: Michele
  full_name: Vendruscolo, Michele
  last_name: Vendruscolo
citation:
  ama: Sinnige T, Ciryam P, Casford S, Dobson CM, de Bono M, Vendruscolo M. Expression
    of the amyloid-β peptide in a single pair of C. elegans sensory neurons modulates
    the associated behavioural response. <i>PLOS ONE</i>. 2019;14(5). doi:<a href="https://doi.org/10.1371/journal.pone.0217746">10.1371/journal.pone.0217746</a>
  apa: Sinnige, T., Ciryam, P., Casford, S., Dobson, C. M., de Bono, M., &#38; Vendruscolo,
    M. (2019). Expression of the amyloid-β peptide in a single pair of C. elegans
    sensory neurons modulates the associated behavioural response. <i>PLOS ONE</i>.
    Public Library of Science. <a href="https://doi.org/10.1371/journal.pone.0217746">https://doi.org/10.1371/journal.pone.0217746</a>
  chicago: Sinnige, Tessa, Prashanth Ciryam, Samuel Casford, Christopher M. Dobson,
    Mario de Bono, and Michele Vendruscolo. “Expression of the Amyloid-β Peptide in
    a Single Pair of C. Elegans Sensory Neurons Modulates the Associated Behavioural
    Response.” <i>PLOS ONE</i>. Public Library of Science, 2019. <a href="https://doi.org/10.1371/journal.pone.0217746">https://doi.org/10.1371/journal.pone.0217746</a>.
  ieee: T. Sinnige, P. Ciryam, S. Casford, C. M. Dobson, M. de Bono, and M. Vendruscolo,
    “Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons
    modulates the associated behavioural response,” <i>PLOS ONE</i>, vol. 14, no.
    5. Public Library of Science, 2019.
  ista: Sinnige T, Ciryam P, Casford S, Dobson CM, de Bono M, Vendruscolo M. 2019.
    Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons
    modulates the associated behavioural response. PLOS ONE. 14(5), e0217746.
  mla: Sinnige, Tessa, et al. “Expression of the Amyloid-β Peptide in a Single Pair
    of C. Elegans Sensory Neurons Modulates the Associated Behavioural Response.”
    <i>PLOS ONE</i>, vol. 14, no. 5, e0217746, Public Library of Science, 2019, doi:<a
    href="https://doi.org/10.1371/journal.pone.0217746">10.1371/journal.pone.0217746</a>.
  short: T. Sinnige, P. Ciryam, S. Casford, C.M. Dobson, M. de Bono, M. Vendruscolo,
    PLOS ONE 14 (2019).
date_created: 2020-02-28T10:45:13Z
date_published: 2019-05-31T00:00:00Z
date_updated: 2021-01-12T08:14:08Z
day: '31'
doi: 10.1371/journal.pone.0217746
extern: '1'
intvolume: '        14'
issue: '5'
language:
- iso: eng
month: '05'
oa_version: Published Version
publication: PLOS ONE
publication_identifier:
  issn:
  - 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: Expression of the amyloid-β peptide in a single pair of C. elegans sensory
  neurons modulates the associated behavioural response
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2019'
...