--- res: bibo_abstract: - Cell polarity is a fundamental feature of all multicellular organisms. In plants, prominent cell polarity markers are PIN auxin transporters crucial for plant development. To identify novel components involved in cell polarity establishment and maintenance, we carried out a forward genetic screening with PIN2:PIN1-HA;pin2 Arabidopsis plants, which ectopically express predominantly basally localized PIN1 in the root epidermal cells leading to agravitropic root growth. From the screen, we identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused PIN1-HA polarity switch from basal to apical side of root epidermal cells. Complementation experiments established the repp12 causative mutation as an amino acid substitution in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase with predicted function in vesicle formation. ala3 T-DNA mutants show defects in many auxin-regulated processes, in asymmetric auxin distribution and in PIN trafficking. Analysis of quintuple and sextuple mutants confirmed a crucial role of ALA proteins in regulating plant development and in PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with GNOM and BIG3 ARF GEFs. Taken together, our results identified ALA3 flippase as an important interactor and regulator of ARF GEF functioning in PIN polarity, trafficking and auxin-mediated development.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Xixi foaf_name: Zhang, Xixi foaf_surname: Zhang foaf_workInfoHomepage: http://www.librecat.org/personId=61A66458-47E9-11EA-85BA-8AEAAF14E49A orcid: 0000-0001-7048-4627 - foaf_Person: foaf_givenName: Maciek foaf_name: Adamowski, Maciek foaf_surname: Adamowski foaf_workInfoHomepage: http://www.librecat.org/personId=45F536D2-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6463-5257 - foaf_Person: foaf_givenName: Petra foaf_name: Marhavá, Petra foaf_surname: Marhavá foaf_workInfoHomepage: http://www.librecat.org/personId=44E59624-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Shutang foaf_name: Tan, Shutang foaf_surname: Tan foaf_workInfoHomepage: http://www.librecat.org/personId=2DE75584-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-0471-8285 - foaf_Person: foaf_givenName: Yuzhou foaf_name: Zhang, Yuzhou foaf_surname: Zhang foaf_workInfoHomepage: http://www.librecat.org/personId=3B6137F2-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2627-6956 - foaf_Person: foaf_givenName: Lesia foaf_name: Rodriguez Solovey, Lesia foaf_surname: Rodriguez Solovey foaf_workInfoHomepage: http://www.librecat.org/personId=3922B506-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-7244-7237 - foaf_Person: foaf_givenName: Marta foaf_name: Zwiewka, Marta foaf_surname: Zwiewka - foaf_Person: foaf_givenName: Vendula foaf_name: Pukyšová, Vendula foaf_surname: Pukyšová - foaf_Person: foaf_givenName: Adrià Sans foaf_name: Sánchez, Adrià Sans foaf_surname: Sánchez - foaf_Person: foaf_givenName: Vivek Kumar foaf_name: Raxwal, Vivek Kumar foaf_surname: Raxwal - foaf_Person: foaf_givenName: Christian S. foaf_name: Hardtke, Christian S. foaf_surname: Hardtke - foaf_Person: foaf_givenName: Tomasz foaf_name: Nodzynski, Tomasz foaf_surname: Nodzynski - foaf_Person: foaf_givenName: Jiří foaf_name: Friml, Jiří foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 bibo_doi: 10.1105/tpc.19.00869 bibo_issue: '5' bibo_volume: 32 dct_date: 2020^xs_gYear dct_identifier: - UT:000545741500030 dct_isPartOf: - http://id.crossref.org/issn/1040-4651 - http://id.crossref.org/issn/1532-298X dct_language: eng dct_publisher: American Society of Plant Biologists@ dct_title: Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters@ fabio_hasPubmedId: '32193204' ...