---
res:
  bibo_abstract:
  - Acute brain slice preparation is a powerful experimental model for investigating
    the characteristics of synaptic function in the brain. Although brain tissue is
    usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal
    damage, exposure to CT causes molecular and architectural changes of synapses.
    To address these issues, we investigated ultrastructural and electrophysiological
    features of synapses in mouse acute cerebellar slices prepared at ice-cold and
    physiological temperature (PT). In the slices prepared at CT, we found significant
    spine loss and reconstruction, synaptic vesicle rearrangement and decrease in
    synaptic proteins, all of which were not detected in slices prepared at PT. Consistent
    with these structural findings, slices prepared at PT showed higher release probability.
    Furthermore, preparation at PT allows electrophysiological recording immediately
    after slicing resulting in higher detectability of long-term depression (LTD)
    after motor learning compared with that at CT. These results indicate substantial
    advantages of the slice preparation at PT for investigating synaptic functions
    in different physiological conditions.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Kohgaku
      foaf_name: Eguchi, Kohgaku
      foaf_surname: Eguchi
      foaf_workInfoHomepage: http://www.librecat.org/personId=2B7846DC-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-6170-2546
  - foaf_Person:
      foaf_givenName: Philipp
      foaf_name: Velicky, Philipp
      foaf_surname: Velicky
      foaf_workInfoHomepage: http://www.librecat.org/personId=39BDC62C-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-2340-7431
  - foaf_Person:
      foaf_givenName: Elena
      foaf_name: Hollergschwandtner, Elena
      foaf_surname: Hollergschwandtner
      foaf_workInfoHomepage: http://www.librecat.org/personId=3C054040-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Makoto
      foaf_name: Itakura, Makoto
      foaf_surname: Itakura
  - foaf_Person:
      foaf_givenName: Yugo
      foaf_name: Fukazawa, Yugo
      foaf_surname: Fukazawa
  - foaf_Person:
      foaf_givenName: Johann G
      foaf_name: Danzl, Johann G
      foaf_surname: Danzl
      foaf_workInfoHomepage: http://www.librecat.org/personId=42EFD3B6-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8559-3973
  - foaf_Person:
      foaf_givenName: Ryuichi
      foaf_name: Shigemoto, Ryuichi
      foaf_surname: Shigemoto
      foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8761-9444
  bibo_doi: 10.3389/fncel.2020.00063
  bibo_volume: 14
  dct_date: 2020^xs_gYear
  dct_identifier:
  - UT:000525582200001
  dct_isPartOf:
  - http://id.crossref.org/issn/1662-5102
  dct_language: eng
  dct_publisher: Frontiers Media@
  dct_title: Advantages of acute brain slices prepared at physiological temperature
    in the characterization of synaptic functions@
  fabio_hasPubmedId: '32265664'
...