article published yes Kohgaku Eguchi author 2B7846DC-F248-11E8-B48F-1D18A9856A870000-0002-6170-2546 Philipp Velicky author 39BDC62C-F248-11E8-B48F-1D18A9856A870000-0002-2340-7431 Elena Hollergschwandtner author 3C054040-F248-11E8-B48F-1D18A9856A87 Makoto Itakura author Yugo Fukazawa author Johann G Danzl author 42EFD3B6-F248-11E8-B48F-1D18A9856A870000-0001-8559-3973 Ryuichi Shigemoto author 499F3ABC-F248-11E8-B48F-1D18A9856A870000-0001-8761-9444 JoDa department RySh department Ultrastructural analysis of phosphoinositides in nerve terminals: distribution, dynamics and physiological roles in synaptic transmission project In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour project Optical control of synaptic function via adhesion molecules project IST Austria Open Access Fund project Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural changes of synapses. To address these issues, we investigated ultrastructural and electrophysiological features of synapses in mouse acute cerebellar slices prepared at ice-cold and physiological temperature (PT). In the slices prepared at CT, we found significant spine loss and reconstruction, synaptic vesicle rearrangement and decrease in synaptic proteins, all of which were not detected in slices prepared at PT. Consistent with these structural findings, slices prepared at PT showed higher release probability. Furthermore, preparation at PT allows electrophysiological recording immediately after slicing resulting in higher detectability of long-term depression (LTD) after motor learning compared with that at CT. These results indicate substantial advantages of the slice preparation at PT for investigating synaptic functions in different physiological conditions. https://research-explorer.ista.ac.at/download/7665/7668/2020_FrontiersCellularNeurosc_Eguchi.pdf application/pdfno Frontiers Media2020 eng 1662-5102 32265664 00052558220000110.3389/fncel.2020.00063 14 Eguchi, Kohgaku, et al. “Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions.” <i>Frontiers in Cellular Neuroscience</i>, vol. 14, 63, Frontiers Media, 2020, doi:<a href="https://doi.org/10.3389/fncel.2020.00063">10.3389/fncel.2020.00063</a>. K. Eguchi <i>et al.</i>, “Advantages of acute brain slices prepared at physiological temperature in the characterization of synaptic functions,” <i>Frontiers in Cellular Neuroscience</i>, vol. 14. Frontiers Media, 2020. K. Eguchi, P. Velicky, E. Saeckl, M. Itakura, Y. Fukazawa, J.G. Danzl, R. Shigemoto, Frontiers in Cellular Neuroscience 14 (2020). Eguchi K, Velicky P, Saeckl E, Itakura M, Fukazawa Y, Danzl JG, Shigemoto R. 2020. Advantages of acute brain slices prepared at physiological temperature in the characterization of synaptic functions. Frontiers in Cellular Neuroscience. 14, 63. Eguchi, K., Velicky, P., Saeckl, E., Itakura, M., Fukazawa, Y., Danzl, J. G., &#38; Shigemoto, R. (2020). Advantages of acute brain slices prepared at physiological temperature in the characterization of synaptic functions. <i>Frontiers in Cellular Neuroscience</i>. Frontiers Media. <a href="https://doi.org/10.3389/fncel.2020.00063">https://doi.org/10.3389/fncel.2020.00063</a> Eguchi K, Velicky P, Saeckl E, et al. Advantages of acute brain slices prepared at physiological temperature in the characterization of synaptic functions. <i>Frontiers in Cellular Neuroscience</i>. 2020;14. doi:<a href="https://doi.org/10.3389/fncel.2020.00063">10.3389/fncel.2020.00063</a> Eguchi, Kohgaku, Philipp Velicky, Elena Saeckl, Makoto Itakura, Yugo Fukazawa, Johann G Danzl, and Ryuichi Shigemoto. “Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions.” <i>Frontiers in Cellular Neuroscience</i>. Frontiers Media, 2020. <a href="https://doi.org/10.3389/fncel.2020.00063">https://doi.org/10.3389/fncel.2020.00063</a>. 76652020-04-19T22:00:55Z2025-06-12T07:16:39Z