---
res:
  bibo_abstract:
  - Following its evoked release, dopamine (DA) signaling is rapidly terminated by
    presynaptic reuptake, mediated by the cocaine-sensitive DA transporter (DAT).
    DAT surface availability is dynamically regulated by endocytic trafficking, and
    direct protein kinase C (PKC) activation acutely diminishes DAT surface expression
    by accelerating DAT internalization. Previous cell line studies demonstrated that
    PKC-stimulated DAT endocytosis requires both Ack1 inactivation, which releases
    a DAT-specific endocytic brake, and the neuronal GTPase, Rit2, which binds DAT.
    However, it is unknown whether Rit2 is required for PKC-stimulated DAT endocytosis
    in DAergic terminals or whether there are region- and/or sex-dependent differences
    in PKC-stimulated DAT trafficking. Moreover, the mechanisms by which Rit2 controls
    PKC-stimulated DAT endocytosis are unknown. Here, we directly examined these important
    questions. Ex vivo studies revealed that PKC activation acutely decreased DAT
    surface expression selectively in ventral, but not dorsal, striatum. AAV-mediated,
    conditional Rit2 knockdown in DAergic neurons impacted baseline DAT surface:intracellular
    distribution in DAergic terminals from female ventral, but not dorsal, striatum.
    Further, Rit2 was required for PKC-stimulated DAT internalization in both male
    and female ventral striatum. FRET and surface pulldown studies in cell lines revealed
    that PKC activation drives DAT-Rit2 surface dissociation and that the DAT N terminus
    is required for both PKC-mediated DAT-Rit2 dissociation and DAT internalization.
    Finally, we found that Rit2 and Ack1 independently converge on DAT to facilitate
    PKC-stimulated DAT endocytosis. Together, our data provide greater insight into
    mechanisms that mediate PKC-regulated DAT internalization and reveal unexpected
    region-specific differences in PKC-stimulated DAT trafficking in bona fide DAergic
    terminals. @eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Rita R.
      foaf_name: Fagan, Rita R.
      foaf_surname: Fagan
  - foaf_Person:
      foaf_givenName: Patrick J.
      foaf_name: Kearney, Patrick J.
      foaf_surname: Kearney
  - foaf_Person:
      foaf_givenName: Carolyn G.
      foaf_name: Sweeney, Carolyn G.
      foaf_surname: Sweeney
  - foaf_Person:
      foaf_givenName: Dino
      foaf_name: Luethi, Dino
      foaf_surname: Luethi
  - foaf_Person:
      foaf_givenName: Florianne E
      foaf_name: Schoot Uiterkamp, Florianne E
      foaf_surname: Schoot Uiterkamp
      foaf_workInfoHomepage: http://www.librecat.org/personId=3526230C-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Klaus
      foaf_name: Schicker, Klaus
      foaf_surname: Schicker
  - foaf_Person:
      foaf_givenName: Brian S.
      foaf_name: Alejandro, Brian S.
      foaf_surname: Alejandro
  - foaf_Person:
      foaf_givenName: Lauren C.
      foaf_name: O'Connor, Lauren C.
      foaf_surname: O'Connor
  - foaf_Person:
      foaf_givenName: Harald H.
      foaf_name: Sitte, Harald H.
      foaf_surname: Sitte
  - foaf_Person:
      foaf_givenName: Haley E.
      foaf_name: Melikian, Haley E.
      foaf_surname: Melikian
  bibo_doi: 10.1074/jbc.RA120.012628
  bibo_issue: '16'
  bibo_volume: 295
  dct_date: 2020^xs_gYear
  dct_identifier:
  - UT:000530288000006
  dct_isPartOf:
  - http://id.crossref.org/issn/0021-9258
  - http://id.crossref.org/issn/1083-351X
  dct_language: eng
  dct_publisher: ASBMB Publications@
  dct_title: 'Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action
    and in vivo impact@'
  fabio_hasPubmedId: '32132171'
...