--- res: bibo_abstract: - Cell migration entails networks and bundles of actin filaments termed lamellipodia and microspikes or filopodia, respectively, as well as focal adhesions, all of which recruit Ena/VASP family members hitherto thought to antagonize efficient cell motility. However, we find these proteins to act as positive regulators of migration in different murine cell lines. CRISPR/Cas9-mediated loss of Ena/VASP proteins reduced lamellipodial actin assembly and perturbed lamellipodial architecture, as evidenced by changed network geometry as well as reduction of filament length and number that was accompanied by abnormal Arp2/3 complex and heterodimeric capping protein accumulation. Loss of Ena/VASP function also abolished the formation of microspikes normally embedded in lamellipodia, but not of filopodia capable of emanating without lamellipodia. Ena/VASP-deficiency also impaired integrin-mediated adhesion accompanied by reduced traction forces exerted through these structures. Our data thus uncover novel Ena/VASP functions of these actin polymerases that are fully consistent with their promotion of cell migration.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Julia foaf_name: Damiano-Guercio, Julia foaf_surname: Damiano-Guercio - foaf_Person: foaf_givenName: Laëtitia foaf_name: Kurzawa, Laëtitia foaf_surname: Kurzawa - foaf_Person: foaf_givenName: Jan foaf_name: Müller, Jan foaf_surname: Müller foaf_workInfoHomepage: http://www.librecat.org/personId=AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D - foaf_Person: foaf_givenName: Georgi A foaf_name: Dimchev, Georgi A foaf_surname: Dimchev foaf_workInfoHomepage: http://www.librecat.org/personId=38C393BE-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8370-6161 - foaf_Person: foaf_givenName: Matthias foaf_name: Schaks, Matthias foaf_surname: Schaks - foaf_Person: foaf_givenName: Maria foaf_name: Nemethova, Maria foaf_surname: Nemethova foaf_workInfoHomepage: http://www.librecat.org/personId=34E27F1C-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Thomas foaf_name: Pokrant, Thomas foaf_surname: Pokrant - foaf_Person: foaf_givenName: Stefan foaf_name: Brühmann, Stefan foaf_surname: Brühmann - foaf_Person: foaf_givenName: Joern foaf_name: Linkner, Joern foaf_surname: Linkner - foaf_Person: foaf_givenName: Laurent foaf_name: Blanchoin, Laurent foaf_surname: Blanchoin - foaf_Person: foaf_givenName: Michael K foaf_name: Sixt, Michael K foaf_surname: Sixt foaf_workInfoHomepage: http://www.librecat.org/personId=41E9FBEA-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-6620-9179 - foaf_Person: foaf_givenName: Klemens foaf_name: Rottner, Klemens foaf_surname: Rottner - foaf_Person: foaf_givenName: Jan foaf_name: Faix, Jan foaf_surname: Faix bibo_doi: 10.7554/eLife.55351 bibo_volume: 9 dct_date: 2020^xs_gYear dct_identifier: - UT:000537208000001 dct_isPartOf: - http://id.crossref.org/issn/2050084X dct_language: eng dct_publisher: eLife Sciences Publications@ dct_title: Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion@ ...