---
_id: '7909'
abstract:
- lang: eng
  text: Cell migration entails networks and bundles of actin filaments termed lamellipodia
    and microspikes or filopodia, respectively, as well as focal adhesions, all of
    which recruit Ena/VASP family members hitherto thought to antagonize efficient
    cell motility. However, we find these proteins to act as positive regulators of
    migration in different murine cell lines. CRISPR/Cas9-mediated loss of Ena/VASP
    proteins reduced lamellipodial actin assembly and perturbed lamellipodial architecture,
    as evidenced by changed network geometry as well as reduction of filament length
    and number that was accompanied by abnormal Arp2/3 complex and heterodimeric capping
    protein accumulation. Loss of Ena/VASP function also abolished the formation of
    microspikes normally embedded in lamellipodia, but not of filopodia capable of
    emanating without lamellipodia. Ena/VASP-deficiency also impaired integrin-mediated
    adhesion accompanied by reduced traction forces exerted through these structures.
    Our data thus uncover novel Ena/VASP functions of these actin polymerases that
    are fully consistent with their promotion of cell migration.
article_number: e55351
article_processing_charge: No
article_type: original
author:
- first_name: Julia
  full_name: Damiano-Guercio, Julia
  last_name: Damiano-Guercio
- first_name: Laëtitia
  full_name: Kurzawa, Laëtitia
  last_name: Kurzawa
- first_name: Jan
  full_name: Müller, Jan
  id: AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D
  last_name: Müller
- first_name: Georgi A
  full_name: Dimchev, Georgi A
  id: 38C393BE-F248-11E8-B48F-1D18A9856A87
  last_name: Dimchev
  orcid: 0000-0001-8370-6161
- first_name: Matthias
  full_name: Schaks, Matthias
  last_name: Schaks
- first_name: Maria
  full_name: Nemethova, Maria
  id: 34E27F1C-F248-11E8-B48F-1D18A9856A87
  last_name: Nemethova
- first_name: Thomas
  full_name: Pokrant, Thomas
  last_name: Pokrant
- first_name: Stefan
  full_name: Brühmann, Stefan
  last_name: Brühmann
- first_name: Joern
  full_name: Linkner, Joern
  last_name: Linkner
- first_name: Laurent
  full_name: Blanchoin, Laurent
  last_name: Blanchoin
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Klemens
  full_name: Rottner, Klemens
  last_name: Rottner
- first_name: Jan
  full_name: Faix, Jan
  last_name: Faix
citation:
  ama: Damiano-Guercio J, Kurzawa L, Müller J, et al. Loss of Ena/VASP interferes
    with lamellipodium architecture, motility and integrin-dependent adhesion. <i>eLife</i>.
    2020;9. doi:<a href="https://doi.org/10.7554/eLife.55351">10.7554/eLife.55351</a>
  apa: Damiano-Guercio, J., Kurzawa, L., Müller, J., Dimchev, G. A., Schaks, M., Nemethova,
    M., … Faix, J. (2020). Loss of Ena/VASP interferes with lamellipodium architecture,
    motility and integrin-dependent adhesion. <i>ELife</i>. eLife Sciences Publications.
    <a href="https://doi.org/10.7554/eLife.55351">https://doi.org/10.7554/eLife.55351</a>
  chicago: Damiano-Guercio, Julia, Laëtitia Kurzawa, Jan Müller, Georgi A Dimchev,
    Matthias Schaks, Maria Nemethova, Thomas Pokrant, et al. “Loss of Ena/VASP Interferes
    with Lamellipodium Architecture, Motility and Integrin-Dependent Adhesion.” <i>ELife</i>.
    eLife Sciences Publications, 2020. <a href="https://doi.org/10.7554/eLife.55351">https://doi.org/10.7554/eLife.55351</a>.
  ieee: J. Damiano-Guercio <i>et al.</i>, “Loss of Ena/VASP interferes with lamellipodium
    architecture, motility and integrin-dependent adhesion,” <i>eLife</i>, vol. 9.
    eLife Sciences Publications, 2020.
  ista: Damiano-Guercio J, Kurzawa L, Müller J, Dimchev GA, Schaks M, Nemethova M,
    Pokrant T, Brühmann S, Linkner J, Blanchoin L, Sixt MK, Rottner K, Faix J. 2020.
    Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent
    adhesion. eLife. 9, e55351.
  mla: Damiano-Guercio, Julia, et al. “Loss of Ena/VASP Interferes with Lamellipodium
    Architecture, Motility and Integrin-Dependent Adhesion.” <i>ELife</i>, vol. 9,
    e55351, eLife Sciences Publications, 2020, doi:<a href="https://doi.org/10.7554/eLife.55351">10.7554/eLife.55351</a>.
  short: J. Damiano-Guercio, L. Kurzawa, J. Müller, G.A. Dimchev, M. Schaks, M. Nemethova,
    T. Pokrant, S. Brühmann, J. Linkner, L. Blanchoin, M.K. Sixt, K. Rottner, J. Faix,
    ELife 9 (2020).
date_created: 2020-05-31T22:00:49Z
date_published: 2020-05-11T00:00:00Z
date_updated: 2026-04-02T14:32:12Z
day: '11'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.7554/eLife.55351
ec_funded: 1
external_id:
  isi:
  - '000537208000001'
  pmid:
  - '32391788'
file:
- access_level: open_access
  checksum: d33bd4441b9a0195718ce1ba5d2c48a6
  content_type: application/pdf
  creator: dernst
  date_created: 2020-06-02T10:35:37Z
  date_updated: 2020-07-14T12:48:05Z
  file_id: '7914'
  file_name: 2020_eLife_Damiano_Guercio.pdf
  file_size: 10535713
  relation: main_file
file_date_updated: 2020-07-14T12:48:05Z
has_accepted_license: '1'
intvolume: '         9'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular Navigation Along Spatial Gradients
publication: eLife
publication_identifier:
  eissn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent
  adhesion
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 9
year: '2020'
...