---
_id: '7941'
abstract:
- lang: eng
  text: Expansion microscopy is a recently developed super-resolution imaging technique,
    which provides an alternative to optics-based methods such as deterministic approaches
    (e.g. STED) or stochastic approaches (e.g. PALM/STORM). The idea behind expansion
    microscopy is to embed the biological sample in a swellable gel, and then to expand
    it isotropically, thereby increasing the distance between the fluorophores. This
    approach breaks the diffraction barrier by simply separating the emission point-spread-functions
    of the fluorophores. The resolution attainable in expansion microscopy is thus
    directly dependent on the separation that can be achieved, i.e. on the expansion
    factor. The original implementation of the technique achieved an expansion factor
    of fourfold, for a resolution of 70–80 nm. The subsequently developed X10 method
    achieves an expansion factor of 10-fold, for a resolution of 25–30 nm. This technique
    can be implemented with minimal technical requirements on any standard fluorescence
    microscope, and is more easily applied for multi-color imaging than either deterministic
    or stochastic super-resolution approaches. This renders X10 expansion microscopy
    a highly promising tool for new biological discoveries, as discussed here, and
    as demonstrated by several recent applications.
article_processing_charge: No
author:
- first_name: Sven M
  full_name: Truckenbrodt, Sven M
  id: 45812BD4-F248-11E8-B48F-1D18A9856A87
  last_name: Truckenbrodt
- first_name: Silvio O.
  full_name: Rizzoli, Silvio O.
  last_name: Rizzoli
citation:
  ama: 'Truckenbrodt SM, Rizzoli SO. Simple multi-color super-resolution by X10 microscopy.
    In: <i>Methods in Cell Biology</i>. Vol 161. Elsevier; 2021:33-56. doi:<a href="https://doi.org/10.1016/bs.mcb.2020.04.016">10.1016/bs.mcb.2020.04.016</a>'
  apa: Truckenbrodt, S. M., &#38; Rizzoli, S. O. (2021). Simple multi-color super-resolution
    by X10 microscopy. In <i>Methods in Cell Biology</i> (Vol. 161, pp. 33–56). Elsevier.
    <a href="https://doi.org/10.1016/bs.mcb.2020.04.016">https://doi.org/10.1016/bs.mcb.2020.04.016</a>
  chicago: Truckenbrodt, Sven M, and Silvio O. Rizzoli. “Simple Multi-Color Super-Resolution
    by X10 Microscopy.” In <i>Methods in Cell Biology</i>, 161:33–56. Elsevier, 2021.
    <a href="https://doi.org/10.1016/bs.mcb.2020.04.016">https://doi.org/10.1016/bs.mcb.2020.04.016</a>.
  ieee: S. M. Truckenbrodt and S. O. Rizzoli, “Simple multi-color super-resolution
    by X10 microscopy,” in <i>Methods in Cell Biology</i>, vol. 161, Elsevier, 2021,
    pp. 33–56.
  ista: 'Truckenbrodt SM, Rizzoli SO. 2021.Simple multi-color super-resolution by
    X10 microscopy. In: Methods in Cell Biology. vol. 161, 33–56.'
  mla: Truckenbrodt, Sven M., and Silvio O. Rizzoli. “Simple Multi-Color Super-Resolution
    by X10 Microscopy.” <i>Methods in Cell Biology</i>, vol. 161, Elsevier, 2021,
    pp. 33–56, doi:<a href="https://doi.org/10.1016/bs.mcb.2020.04.016">10.1016/bs.mcb.2020.04.016</a>.
  short: S.M. Truckenbrodt, S.O. Rizzoli, in:, Methods in Cell Biology, Elsevier,
    2021, pp. 33–56.
corr_author: '1'
date_created: 2020-06-07T22:00:55Z
date_published: 2021-01-01T00:00:00Z
date_updated: 2024-10-09T20:59:36Z
day: '01'
department:
- _id: JoDa
doi: 10.1016/bs.mcb.2020.04.016
external_id:
  pmid:
  - '33478696'
intvolume: '       161'
language:
- iso: eng
month: '01'
oa_version: None
page: 33-56
pmid: 1
publication: Methods in Cell Biology
publication_identifier:
  isbn:
  - 978012820807-6
  issn:
  - 0091-679X
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Simple multi-color super-resolution by X10 microscopy
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 161
year: '2021'
...