---
_id: '805'
abstract:
- lang: eng
  text: During corticogenesis, distinct classes of neurons are born from progenitor
    cells located in the ventricular and subventricular zones, from where they migrate
    towards the pial surface to assemble into highly organized layer-specific circuits.
    However, the precise and coordinated transcriptional network activity defining
    neuronal identity is still not understood. Here, we show that genetic depletion
    of the basic helix-loop-helix (bHLH) transcription factor E2A splice variant E47
    increased the number of Tbr1-positive deep layer and Satb2-positive upper layer
    neurons at E14.5, while depletion of the alternatively spliced E12 variant did
    not affect layer-specific neurogenesis. While ChIP-Seq identified a big overlap
    for E12- and E47-specific binding sites in embryonic NSCs, including sites at
    the cyclin-dependent kinase inhibitor (CDKI) Cdkn1c gene locus, RNA-Seq revealed
    a unique transcriptional regulation by each splice variant. E47 activated the
    expression of the CDKI Cdkn1c through binding to a distal enhancer. Finally, overexpression
    of E47 in embryonic NSCs in vitro impaired neurite outgrowth and E47 overexpression
    in vivo by in utero electroporation disturbed proper layer-specific neurogenesis
    and upregulated p57(KIP2) expression. Overall, this study identified E2A target
    genes in embryonic NSCs and demonstrates that E47 regulates neuronal differentiation
    via p57(KIP2).
article_processing_charge: No
author:
- first_name: Sabrina
  full_name: Pfurr, Sabrina
  last_name: Pfurr
- first_name: Yu
  full_name: Chu, Yu
  last_name: Chu
- first_name: Christian
  full_name: Bohrer, Christian
  last_name: Bohrer
- first_name: Franziska
  full_name: Greulich, Franziska
  last_name: Greulich
- first_name: Robert J
  full_name: Beattie, Robert J
  id: 2E26DF60-F248-11E8-B48F-1D18A9856A87
  last_name: Beattie
  orcid: 0000-0002-8483-8753
- first_name: Könül
  full_name: Mammadzada, Könül
  last_name: Mammadzada
- first_name: Miriam
  full_name: Hils, Miriam
  last_name: Hils
- first_name: Sebastian
  full_name: Arnold, Sebastian
  last_name: Arnold
- first_name: Verdon
  full_name: Taylor, Verdon
  last_name: Taylor
- first_name: Kristina
  full_name: Schachtrup, Kristina
  last_name: Schachtrup
- first_name: N Henriette
  full_name: Uhlenhaut, N Henriette
  last_name: Uhlenhaut
- first_name: Christian
  full_name: Schachtrup, Christian
  last_name: Schachtrup
citation:
  ama: Pfurr S, Chu Y, Bohrer C, et al. The E2A splice variant E47 regulates the differentiation
    of projection neurons via p57(KIP2) during cortical development. <i>Development</i>.
    2017;144:3917-3931. doi:<a href="https://doi.org/10.1242/dev.145698">10.1242/dev.145698</a>
  apa: Pfurr, S., Chu, Y., Bohrer, C., Greulich, F., Beattie, R. J., Mammadzada, K.,
    … Schachtrup, C. (2017). The E2A splice variant E47 regulates the differentiation
    of projection neurons via p57(KIP2) during cortical development. <i>Development</i>.
    Company of Biologists. <a href="https://doi.org/10.1242/dev.145698">https://doi.org/10.1242/dev.145698</a>
  chicago: Pfurr, Sabrina, Yu Chu, Christian Bohrer, Franziska Greulich, Robert J
    Beattie, Könül Mammadzada, Miriam Hils, et al. “The E2A Splice Variant E47 Regulates
    the Differentiation of Projection Neurons via P57(KIP2) during Cortical Development.”
    <i>Development</i>. Company of Biologists, 2017. <a href="https://doi.org/10.1242/dev.145698">https://doi.org/10.1242/dev.145698</a>.
  ieee: S. Pfurr <i>et al.</i>, “The E2A splice variant E47 regulates the differentiation
    of projection neurons via p57(KIP2) during cortical development,” <i>Development</i>,
    vol. 144. Company of Biologists, pp. 3917–3931, 2017.
  ista: Pfurr S, Chu Y, Bohrer C, Greulich F, Beattie RJ, Mammadzada K, Hils M, Arnold
    S, Taylor V, Schachtrup K, Uhlenhaut NH, Schachtrup C. 2017. The E2A splice variant
    E47 regulates the differentiation of projection neurons via p57(KIP2) during cortical
    development. Development. 144, 3917–3931.
  mla: Pfurr, Sabrina, et al. “The E2A Splice Variant E47 Regulates the Differentiation
    of Projection Neurons via P57(KIP2) during Cortical Development.” <i>Development</i>,
    vol. 144, Company of Biologists, 2017, pp. 3917–31, doi:<a href="https://doi.org/10.1242/dev.145698">10.1242/dev.145698</a>.
  short: S. Pfurr, Y. Chu, C. Bohrer, F. Greulich, R.J. Beattie, K. Mammadzada, M.
    Hils, S. Arnold, V. Taylor, K. Schachtrup, N.H. Uhlenhaut, C. Schachtrup, Development
    144 (2017) 3917–3931.
date_created: 2018-12-11T11:48:36Z
date_published: 2017-10-31T00:00:00Z
date_updated: 2023-09-26T16:20:09Z
day: '31'
department:
- _id: SiHi
doi: 10.1242/dev.145698
external_id:
  isi:
  - '000414025600007'
intvolume: '       144'
isi: 1
language:
- iso: eng
month: '10'
oa_version: None
page: 3917 - 3931
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '6846'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The E2A splice variant E47 regulates the differentiation of projection neurons
  via p57(KIP2) during cortical development
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 144
year: '2017'
...