---
_id: '810'
abstract:
- lang: eng
  text: Cryo-electron tomography combined with image processing by sub-tomogram averaging
    is unique in its power to resolve the structures of proteins and macromolecular
    complexes in situ. Limitations of the method, including the low signal to noise
    ratio within individual images from cryo-tomographic datasets and difficulties
    in determining the defocus at which the data was collected, mean that to date
    the very best structures obtained by sub-tomogram averaging are limited to a resolution
    of approximately 15. Å. Here, by optimizing data collection and defocus determination
    steps, we have determined the structure of assembled Mason-Pfizer monkey virus
    Gag protein using sub-tomogram averaging to a resolution of 8.5. Å. At this resolution
    alpha-helices can be directly and clearly visualized. These data demonstrate for
    the first time that high-resolution structural information can be obtained from
    cryo-electron tomograms using sub-tomogram averaging. Sub-tomogram averaging has
    the potential to allow detailed studies of unsolved and biologically relevant
    structures under biologically relevant conditions.
acknowledgement: The M-PMV ΔPro CANC tubes imaged in this study were a kind gift from
  Pavel Ulbrich and Tomas Ruml, Institute of Chemical Technology, Prague. The cryo-EM
  grids were prepared by Tanmay Bharat. This study was technically supported by EMBL’s
  IT services unit and by Frank Thommen. We thank Martin Schorb and Svetlana Dodonova
  for discussions and advice; Khanh Huy Bui for advice and scripts to streamline tomogram
  reconstruction; and Giulia Zanetti, Tanmay Bharat, and Martin Beck for comments
  on the manuscript. This study was supported by Deutsche Forschungsgemeinschaft grant
  BR 3635/2-1 to JAGB.
author:
- first_name: Florian
  full_name: Florian Schur
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
- first_name: Wim
  full_name: Hagen, Wim J
  last_name: Hagen
- first_name: Alex
  full_name: De Marco, Alex
  last_name: De Marco
- first_name: John
  full_name: Briggs, John A
  last_name: Briggs
citation:
  ama: Schur FK, Hagen W, De Marco A, Briggs J. Determination of protein structure
    at 8.5Å resolution using cryo-electron tomography and sub-tomogram averaging.
    <i>Journal of Structural Biology</i>. 2013;184(3):394-400. doi:<a href="https://doi.org/10.1016/j.jsb.2013.10.015">10.1016/j.jsb.2013.10.015</a>
  apa: Schur, F. K., Hagen, W., De Marco, A., &#38; Briggs, J. (2013). Determination
    of protein structure at 8.5Å resolution using cryo-electron tomography and sub-tomogram
    averaging. <i>Journal of Structural Biology</i>. Academic Press. <a href="https://doi.org/10.1016/j.jsb.2013.10.015">https://doi.org/10.1016/j.jsb.2013.10.015</a>
  chicago: Schur, Florian KM, Wim Hagen, Alex De Marco, and John Briggs. “Determination
    of Protein Structure at 8.5Å Resolution Using Cryo-Electron Tomography and Sub-Tomogram
    Averaging.” <i>Journal of Structural Biology</i>. Academic Press, 2013. <a href="https://doi.org/10.1016/j.jsb.2013.10.015">https://doi.org/10.1016/j.jsb.2013.10.015</a>.
  ieee: F. K. Schur, W. Hagen, A. De Marco, and J. Briggs, “Determination of protein
    structure at 8.5Å resolution using cryo-electron tomography and sub-tomogram averaging,”
    <i>Journal of Structural Biology</i>, vol. 184, no. 3. Academic Press, pp. 394–400,
    2013.
  ista: Schur FK, Hagen W, De Marco A, Briggs J. 2013. Determination of protein structure
    at 8.5Å resolution using cryo-electron tomography and sub-tomogram averaging.
    Journal of Structural Biology. 184(3), 394–400.
  mla: Schur, Florian KM, et al. “Determination of Protein Structure at 8.5Å Resolution
    Using Cryo-Electron Tomography and Sub-Tomogram Averaging.” <i>Journal of Structural
    Biology</i>, vol. 184, no. 3, Academic Press, 2013, pp. 394–400, doi:<a href="https://doi.org/10.1016/j.jsb.2013.10.015">10.1016/j.jsb.2013.10.015</a>.
  short: F.K. Schur, W. Hagen, A. De Marco, J. Briggs, Journal of Structural Biology
    184 (2013) 394–400.
date_created: 2018-12-11T11:48:37Z
date_published: 2013-12-01T00:00:00Z
date_updated: 2021-01-12T08:16:54Z
day: '01'
doi: 10.1016/j.jsb.2013.10.015
extern: 1
intvolume: '       184'
issue: '3'
month: '12'
page: 394 - 400
publication: Journal of Structural Biology
publication_status: published
publisher: Academic Press
publist_id: '6839'
quality_controlled: 0
status: public
title: Determination of protein structure at 8.5Å resolution using cryo-electron tomography
  and sub-tomogram averaging
type: journal_article
volume: 184
year: '2013'
...