---
res:
  bibo_abstract:
  - The Gag polyprotein of retroviruses drives immature virus assembly by forming
    hexameric protein lattices. The assembly is primarily mediated by protein-protein
    interactions between capsid (CA) domains and by interactions between nucleocapsid
    (NC) domains and RNA. Specific interactions between NC and the viral RNA are required
    for genome packaging. Previously reported cryoelectron microscopy analysis of
    immature Mason-Pfizer monkey virus (M-PMV) particles suggested that a basic region
    (residues RKK) in CA may serve as an additional binding site for nucleic acids.
    Here, we have introduced mutations into the RKK region in both bacterial and proviral
    M-PMV vectors and have assessed their impact on M-PMV assembly, structure, RNA
    binding, budding/release, nuclear trafficking, and infectivity using in vitro
    and in vivo systems. Our data indicate that the RKK region binds and structures
    nucleic acid that serves to promote virus particle assembly in the cytoplasm.
    Moreover, the RKK region appears to be important for recruitment of viral genomic
    RNA into Gag particles, and this function could be linked to changes in nuclear
    trafficking. Together these observations suggest that in M-PMV, direct interactions
    between CA and nucleic acid play important functions in the late stages of the
    viral life cycle.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Tibor
      foaf_name: Füzik, Tibor
      foaf_surname: Füzik
  - foaf_Person:
      foaf_givenName: Růžena
      foaf_name: Píchalová, Růžena
      foaf_surname: Píchalová
  - foaf_Person:
      foaf_givenName: Florian
      foaf_name: Schur, Florian
      foaf_surname: Schur
      foaf_workInfoHomepage: http://www.librecat.org/personId=48AD8942-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-4790-8078
  - foaf_Person:
      foaf_givenName: Karolína
      foaf_name: Strohalmová, Karolína
      foaf_surname: Strohalmová
  - foaf_Person:
      foaf_givenName: Ivana
      foaf_name: Křížová, Ivana
      foaf_surname: Křížová
  - foaf_Person:
      foaf_givenName: Romana
      foaf_name: Hadravová, Romana
      foaf_surname: Hadravová
  - foaf_Person:
      foaf_givenName: Michaela
      foaf_name: Rumlová, Michaela
      foaf_surname: Rumlová
  - foaf_Person:
      foaf_givenName: John
      foaf_name: Briggs, John
      foaf_surname: Briggs
  - foaf_Person:
      foaf_givenName: Pavel
      foaf_name: Ulbrich, Pavel
      foaf_surname: Ulbrich
  - foaf_Person:
      foaf_givenName: Tomáš
      foaf_name: Ruml, Tomáš
      foaf_surname: Ruml
  bibo_doi: 10.1128/JVI.03197-15
  bibo_issue: '9'
  bibo_volume: 90
  dct_date: 2016^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0022-538X
  - http://id.crossref.org/issn/1098-5514
  dct_language: eng
  dct_publisher: American Society for Microbiology@
  dct_title: Nucleic acid binding by Mason-Pfizer monkey virus CA promotes virus assembly
    and genome packaging@
  fabio_hasPubmedId: '26912613'
...