---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '813'
abstract:
- lang: eng
  text: The Gag polyprotein of retroviruses drives immature virus assembly by forming
    hexameric protein lattices. The assembly is primarily mediated by protein-protein
    interactions between capsid (CA) domains and by interactions between nucleocapsid
    (NC) domains and RNA. Specific interactions between NC and the viral RNA are required
    for genome packaging. Previously reported cryoelectron microscopy analysis of
    immature Mason-Pfizer monkey virus (M-PMV) particles suggested that a basic region
    (residues RKK) in CA may serve as an additional binding site for nucleic acids.
    Here, we have introduced mutations into the RKK region in both bacterial and proviral
    M-PMV vectors and have assessed their impact on M-PMV assembly, structure, RNA
    binding, budding/release, nuclear trafficking, and infectivity using in vitro
    and in vivo systems. Our data indicate that the RKK region binds and structures
    nucleic acid that serves to promote virus particle assembly in the cytoplasm.
    Moreover, the RKK region appears to be important for recruitment of viral genomic
    RNA into Gag particles, and this function could be linked to changes in nuclear
    trafficking. Together these observations suggest that in M-PMV, direct interactions
    between CA and nucleic acid play important functions in the late stages of the
    viral life cycle.
acknowledgement: "Work in the laboratory of John A. G. Briggs was funded by Deutsche\r\nForschungsgemeinschaft
  (DFG) (BR 3635/2-1). This work, including the\r\nefforts of Tomas Ruml, was funded
  by the Grant Agency of the Czech\r\nRepublic (14-15326S) and the Czech Ministry
  of Education (NPU I sus-\r\ntainability projects LO1302 and LO1304)."
article_processing_charge: No
article_type: original
author:
- first_name: Tibor
  full_name: Füzik, Tibor
  last_name: Füzik
- first_name: Růžena
  full_name: Píchalová, Růžena
  last_name: Píchalová
- first_name: Florian
  full_name: Schur, Florian
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
- first_name: Karolína
  full_name: Strohalmová, Karolína
  last_name: Strohalmová
- first_name: Ivana
  full_name: Křížová, Ivana
  last_name: Křížová
- first_name: Romana
  full_name: Hadravová, Romana
  last_name: Hadravová
- first_name: Michaela
  full_name: Rumlová, Michaela
  last_name: Rumlová
- first_name: John
  full_name: Briggs, John
  last_name: Briggs
- first_name: Pavel
  full_name: Ulbrich, Pavel
  last_name: Ulbrich
- first_name: Tomáš
  full_name: Ruml, Tomáš
  last_name: Ruml
citation:
  ama: Füzik T, Píchalová R, Schur FK, et al. Nucleic acid binding by Mason-Pfizer
    monkey virus CA promotes virus assembly and genome packaging. <i>Journal of Virology</i>.
    2016;90(9):4593-4603. doi:<a href="https://doi.org/10.1128/JVI.03197-15">10.1128/JVI.03197-15</a>
  apa: Füzik, T., Píchalová, R., Schur, F. K., Strohalmová, K., Křížová, I., Hadravová,
    R., … Ruml, T. (2016). Nucleic acid binding by Mason-Pfizer monkey virus CA promotes
    virus assembly and genome packaging. <i>Journal of Virology</i>. American Society
    for Microbiology. <a href="https://doi.org/10.1128/JVI.03197-15">https://doi.org/10.1128/JVI.03197-15</a>
  chicago: Füzik, Tibor, Růžena Píchalová, Florian KM Schur, Karolína Strohalmová,
    Ivana Křížová, Romana Hadravová, Michaela Rumlová, John Briggs, Pavel Ulbrich,
    and Tomáš Ruml. “Nucleic Acid Binding by Mason-Pfizer Monkey Virus CA Promotes
    Virus Assembly and Genome Packaging.” <i>Journal of Virology</i>. American Society
    for Microbiology, 2016. <a href="https://doi.org/10.1128/JVI.03197-15">https://doi.org/10.1128/JVI.03197-15</a>.
  ieee: T. Füzik <i>et al.</i>, “Nucleic acid binding by Mason-Pfizer monkey virus
    CA promotes virus assembly and genome packaging,” <i>Journal of Virology</i>,
    vol. 90, no. 9. American Society for Microbiology, pp. 4593–4603, 2016.
  ista: Füzik T, Píchalová R, Schur FK, Strohalmová K, Křížová I, Hadravová R, Rumlová
    M, Briggs J, Ulbrich P, Ruml T. 2016. Nucleic acid binding by Mason-Pfizer monkey
    virus CA promotes virus assembly and genome packaging. Journal of Virology. 90(9),
    4593–4603.
  mla: Füzik, Tibor, et al. “Nucleic Acid Binding by Mason-Pfizer Monkey Virus CA
    Promotes Virus Assembly and Genome Packaging.” <i>Journal of Virology</i>, vol.
    90, no. 9, American Society for Microbiology, 2016, pp. 4593–603, doi:<a href="https://doi.org/10.1128/JVI.03197-15">10.1128/JVI.03197-15</a>.
  short: T. Füzik, R. Píchalová, F.K. Schur, K. Strohalmová, I. Křížová, R. Hadravová,
    M. Rumlová, J. Briggs, P. Ulbrich, T. Ruml, Journal of Virology 90 (2016) 4593–4603.
date_created: 2018-12-11T11:48:38Z
date_published: 2016-05-01T00:00:00Z
date_updated: 2026-05-20T07:34:00Z
day: '01'
doi: 10.1128/JVI.03197-15
extern: '1'
external_id:
  pmid:
  - '26912613'
intvolume: '        90'
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1128/jvi.03197-15
month: '05'
oa: 1
oa_version: Published Version
page: 4593 - 4603
pmid: 1
publication: Journal of Virology
publication_identifier:
  eissn:
  - 1098-5514
  issn:
  - 0022-538X
publication_status: published
publisher: American Society for Microbiology
publist_id: '6835'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Nucleic acid binding by Mason-Pfizer monkey virus CA promotes virus assembly
  and genome packaging
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 90
year: '2016'
...