---
res:
  bibo_abstract:
  - Human immunodeficiency virus type 1 (HIV-1) assembly proceeds in two stages. First,
    the 55 kilodalton viral Gag polyprotein assembles into a hexameric protein lattice
    at the plasma membrane of the infected cell, inducing budding and release of an
    immature particle. Second, Gag is cleaved by the viral protease, leading to internal
    rearrangement of the virus into the mature, infectious form. Immature and mature
    HIV-1 particles are heterogeneous in size and morphology, preventing high-resolution
    analysis of their protein arrangement in situ by conventional structural biology
    methods. Here we apply cryo-electron tomography and sub-tomogram averaging methods
    to resolve the structure of the capsid lattice within intact immature HIV-1 particles
    at subnanometre resolution, allowing unambiguous positioning of all α-helices.
    The resulting model reveals tertiary and quaternary structural interactions that
    mediate HIV-1 assembly. Strikingly, these interactions differ from those predicted
    by the current model based on in vitro-assembled arrays of Gag-derived proteins
    from Mason-Pfizer monkey virus. To validate this difference, we solve the structure
    of the capsid lattice within intact immature Mason-Pfizer monkey virus particles.
    Comparison with the immature HIV-1 structure reveals that retroviral capsid proteins,
    while having conserved tertiary structures, adopt different quaternary arrangements
    during virus assembly. The approach demonstrated here should be applicable to
    determine structures of other proteins at subnanometre resolution within heterogeneous
    environments.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Florian
      foaf_name: Schur, Florian
      foaf_surname: Schur
      foaf_workInfoHomepage: http://www.librecat.org/personId=48AD8942-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-4790-8078
  - foaf_Person:
      foaf_givenName: Wim
      foaf_name: Hagen, Wim
      foaf_surname: Hagen
  - foaf_Person:
      foaf_givenName: Michaela
      foaf_name: Rumlová, Michaela
      foaf_surname: Rumlová
  - foaf_Person:
      foaf_givenName: Tomáš
      foaf_name: Ruml, Tomáš
      foaf_surname: Ruml
  - foaf_Person:
      foaf_givenName: B
      foaf_name: Müller, B
      foaf_surname: Müller
  - foaf_Person:
      foaf_givenName: Hans
      foaf_name: Kraüsslich, Hans
      foaf_surname: Kraüsslich
  - foaf_Person:
      foaf_givenName: John
      foaf_name: Briggs, John
      foaf_surname: Briggs
  bibo_doi: 10.1038/nature13838
  bibo_issue: '7535'
  bibo_volume: 517
  dct_date: 2015^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0028-0836
  - http://id.crossref.org/issn/1476-4687
  dct_language: eng
  dct_publisher: Springer Nature@
  dct_title: Structure of the immature HIV-1 capsid in intact virus particles at 8.8
    Å resolution@
  fabio_hasPubmedId: '25363765'
...