article published yes Juan C Montesinos López author 310A8E3E-F248-11E8-B48F-1D18A9856A870000-0001-9179-6099 A Abuzeineh author Aglaja Kopf author 31DAC7B6-F248-11E8-B48F-1D18A9856A870000-0002-2187-6656 Alba Juanes Garcia author 40F05888-F248-11E8-B48F-1D18A9856A870000-0002-1009-9652 Krisztina Ötvös author 29B901B0-F248-11E8-B48F-1D18A9856A870000-0002-5503-4983 J Petrášek author Michael K Sixt author 41E9FBEA-F248-11E8-B48F-1D18A9856A870000-0002-6620-9179 Eva Benková author 38F4F166-F248-11E8-B48F-1D18A9856A870000-0002-8510-9739 MiSi department EvBe department Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants project Hormone cross-talk drives nutrient dependent plant development project Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells. https://research-explorer.ista.ac.at/download/8142/8827/2020_EMBO_Montesinos.pdf application/pdfno Embo Press2020 eng 0261-4189 1460-2075 32667089 00054831180000110.15252/embj.2019104238 3917 Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. The Embo Journal. 39(17), e104238. Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. 2020;39(17). doi:<a href="https://doi.org/10.15252/embj.2019104238">10.15252/embj.2019104238</a> J. C. Montesinos López <i>et al.</i>, “Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage,” <i>The Embo Journal</i>, vol. 39, no. 17. Embo Press, 2020. J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös, J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020). Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia, Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>. Embo Press, 2020. <a href="https://doi.org/10.15252/embj.2019104238">https://doi.org/10.15252/embj.2019104238</a>. Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös, K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. Embo Press. <a href="https://doi.org/10.15252/embj.2019104238">https://doi.org/10.15252/embj.2019104238</a> Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>, vol. 39, no. 17, e104238, Embo Press, 2020, doi:<a href="https://doi.org/10.15252/embj.2019104238">10.15252/embj.2019104238</a>. 81422020-07-21T09:08:38Z2025-04-15T06:37:27Z