---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '815'
abstract:
- lang: eng
  text: "The polyprotein Gag is the primary structural component of retroviruses.
    Gag consists of independently folded domains connected by flexible linkers. Interactions
    between the conserved capsid (CA) domains of Gag mediate formation of hexameric
    protein lattices that drive assembly of immature virus particles. Proteolytic
    cleavage of Gag by the viral protease (PR) is required for maturation of retroviruses
    from an immature form into an infectious form. Within the assembled Gag lattices
    of HIV-1 and Mason- Pfizer monkey virus (M-PMV), the C-terminal domain of CA adopts
    similar quaternary arrangements, while the N-terminal domain of CA is packed in
    very different manners. Here, we have used cryo-electron tomography and subtomogram
    averaging to study in vitro-assembled, immature virus-like Rous sarcoma virus
    (RSV) Gag particles and have determined the structure of CA and the surrounding
    regions to a resolution of ~8 Å. We found that the C-terminal domain of RSV CA
    is arranged similarly to HIV-1 and M-PMV, whereas the N-terminal domain of CA
    adopts a novel arrangement in which the upstream p10 domain folds back into the
    CA lattice. In this position the cleavage site between CA and p10 appears to be
    inaccessible to PR. Below CA, an extended density is consistent with the presence
    of a six-helix bundle formed by the spacer-peptide region. We have also assessed
    the affect of lattice assembly on proteolytic processing by exogenous PR. The
    cleavage between p10 and CA is indeed inhibited in the assembled lattice, a finding
    consistent with structural regulation of proteolytic maturation.\r\n"
article_processing_charge: No
article_type: original
author:
- first_name: Florian
  full_name: Schur, Florian
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
- first_name: Robert
  full_name: Dick, Robert
  last_name: Dick
- first_name: Wim
  full_name: Hagen, Wim
  last_name: Hagen
- first_name: Volker
  full_name: Vogt, Volker
  last_name: Vogt
- first_name: John
  full_name: Briggs, John
  last_name: Briggs
citation:
  ama: Schur FK, Dick R, Hagen W, Vogt V, Briggs J. The structure of immature virus
    like Rous sarcoma virus gag particles reveals a structural role for the p10 domain
    in assembly. <i>Journal of Virology</i>. 2015;89(20):10294-10302. doi:<a href="https://doi.org/10.1128/JVI.01502-15">10.1128/JVI.01502-15</a>
  apa: Schur, F. K., Dick, R., Hagen, W., Vogt, V., &#38; Briggs, J. (2015). The structure
    of immature virus like Rous sarcoma virus gag particles reveals a structural role
    for the p10 domain in assembly. <i>Journal of Virology</i>. ASM. <a href="https://doi.org/10.1128/JVI.01502-15">https://doi.org/10.1128/JVI.01502-15</a>
  chicago: Schur, Florian KM, Robert Dick, Wim Hagen, Volker Vogt, and John Briggs.
    “The Structure of Immature Virus like Rous Sarcoma Virus Gag Particles Reveals
    a Structural Role for the P10 Domain in Assembly.” <i>Journal of Virology</i>.
    ASM, 2015. <a href="https://doi.org/10.1128/JVI.01502-15">https://doi.org/10.1128/JVI.01502-15</a>.
  ieee: F. K. Schur, R. Dick, W. Hagen, V. Vogt, and J. Briggs, “The structure of
    immature virus like Rous sarcoma virus gag particles reveals a structural role
    for the p10 domain in assembly,” <i>Journal of Virology</i>, vol. 89, no. 20.
    ASM, pp. 10294–10302, 2015.
  ista: Schur FK, Dick R, Hagen W, Vogt V, Briggs J. 2015. The structure of immature
    virus like Rous sarcoma virus gag particles reveals a structural role for the
    p10 domain in assembly. Journal of Virology. 89(20), 10294–10302.
  mla: Schur, Florian KM, et al. “The Structure of Immature Virus like Rous Sarcoma
    Virus Gag Particles Reveals a Structural Role for the P10 Domain in Assembly.”
    <i>Journal of Virology</i>, vol. 89, no. 20, ASM, 2015, pp. 10294–302, doi:<a
    href="https://doi.org/10.1128/JVI.01502-15">10.1128/JVI.01502-15</a>.
  short: F.K. Schur, R. Dick, W. Hagen, V. Vogt, J. Briggs, Journal of Virology 89
    (2015) 10294–10302.
date_created: 2018-12-11T11:48:39Z
date_published: 2015-09-22T00:00:00Z
date_updated: 2026-05-18T12:47:51Z
day: '22'
doi: 10.1128/JVI.01502-15
extern: '1'
external_id:
  pmid:
  - '26223638'
intvolume: '        89'
issue: '20'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1128/jvi.01502-15
month: '09'
oa: 1
oa_version: Published Version
page: 10294 - 10302
pmid: 1
publication: Journal of Virology
publication_identifier:
  eissn:
  - 1098-5514
  issn:
  - 0022-538X
publication_status: published
publisher: ASM
publist_id: '6837'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The structure of immature virus like Rous sarcoma virus gag particles reveals
  a structural role for the p10 domain in assembly
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 89
year: '2015'
...