report published Florian Schur author 48AD8942-F248-11E8-B48F-1D18A9856A870000-0003-4790-8078 Martin Obr author 4741CA5A-F248-11E8-B48F-1D18A9856A870000-0003-1756-6564 Wim Hagen author William Wan author Arjen Jakobi author Joanna Kirkpatrick author Carsten Sachse author Hans Kraüsslich author John Briggs author Immature HIV-1 assembles at and buds from the plasma membrane before proteolytic cleavage of the viral Gag polyprotein induces structural maturation. Maturation can be blocked by maturation inhibitors (MIs), thereby abolishing infectivity. The CA (capsid) and SP1 (spacer peptide 1) region of Gag is the key regulator of assembly and maturation and is the target of MIs.We applied optimized cryo-electron tomography and subtomogram averaging to resolve this region within assembled immature HIV-1 particles at 3.9 angstrom resolution and built an atomic model. The structure reveals a network of intra- And intermolecular interactions mediating immature HIV-1 assembly. The proteolytic cleavage site between CA and SP1 is inaccessible to protease.We suggest that MIs prevent CA-SP1 cleavage by stabilizing the structure, and MI resistance develops by destabilizing CA-SP1. American Association for the Advancement of Science2016 eng 0036-8075 1095-9203 2741749710.1126/science.aaf9620 353506 - 508 yes Schur, Florian KM, Martin Obr, Wim Hagen, William Wan, Arjen Jakobi, Joanna Kirkpatrick, Carsten Sachse, Hans Kraüsslich, and John Briggs. <i>An Atomic Model of HIV-1 Capsid-SP1 Reveals Structures Regulating Assembly and Maturation</i>. Vol. 353. American Association for the Advancement of Science, 2016. <a href="https://doi.org/10.1126/science.aaf9620">https://doi.org/10.1126/science.aaf9620</a>. Schur, Florian KM, et al. <i>An Atomic Model of HIV-1 Capsid-SP1 Reveals Structures Regulating Assembly and Maturation</i>. Vol. 353, American Association for the Advancement of Science, 2016, pp. 506–08, doi:<a href="https://doi.org/10.1126/science.aaf9620">10.1126/science.aaf9620</a>. Schur FK, Obr M, Hagen W, et al. <i>An Atomic Model of HIV-1 Capsid-SP1 Reveals Structures Regulating Assembly and Maturation</i>. Vol 353. American Association for the Advancement of Science; 2016:506-508. doi:<a href="https://doi.org/10.1126/science.aaf9620">10.1126/science.aaf9620</a> Schur, F. K., Obr, M., Hagen, W., Wan, W., Jakobi, A., Kirkpatrick, J., … Briggs, J. (2016). <i>An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation</i> (Vol. 353, pp. 506–508). American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.aaf9620">https://doi.org/10.1126/science.aaf9620</a> F. K. Schur <i>et al.</i>, <i>An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation</i>, vol. 353. American Association for the Advancement of Science, 2016, pp. 506–508. F.K. Schur, M. Obr, W. Hagen, W. Wan, A. Jakobi, J. Kirkpatrick, C. Sachse, H. Kraüsslich, J. Briggs, An Atomic Model of HIV-1 Capsid-SP1 Reveals Structures Regulating Assembly and Maturation, American Association for the Advancement of Science, 2016. Schur FK, Obr M, Hagen W, Wan W, Jakobi A, Kirkpatrick J, Sachse C, Kraüsslich H, Briggs J. 2016. An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation, American Association for the Advancement of Science,p. 8162018-12-11T11:48:39Z2026-05-20T07:41:16Z