---
res:
  bibo_abstract:
  - Cryo-electron tomography (cryo-ET) allows cellular ultrastructures and macromolecular
    complexes to be imaged in three-dimensions in their native environments. Cryo-electron
    tomograms are reconstructed from projection images taken at defined tilt-angles.
    In order to recover high-resolution information from cryo-electron tomograms,
    it is necessary to measure and correct for the contrast transfer function (CTF)
    of the microscope. Most commonly, this is performed using protocols that approximate
    the sample as a two-dimensional (2D) plane. This approximation accounts for differences
    in defocus and therefore CTF across the tilted sample. It does not account for
    differences in defocus of objects at different heights within the sample; instead,
    a 3D approach is required. Currently available approaches for 3D-CTF correction
    are computationally expensive and have not been widely implemented. Here we simulate
    the benefits of 3D-CTF correction for high-resolution subtomogram averaging, and
    present a user-friendly, computationally-efficient 3D-CTF correction tool, NovaCTF,
    that is compatible with standard tomogram reconstruction workflows in IMOD. We
    validate the approach on synthetic data and test it using subtomogram averaging
    of real data. Consistent with our simulations, we find that 3D-CTF correction
    allows high-resolution structures to be obtained with much smaller subtomogram
    averaging datasets than are required using 2D-CTF. We also show that using equivalent
    dataset sizes, 3D-CTF correction can be used to obtain higher-resolution structures.
    We present a 3.4. Å resolution structure determined by subtomogram averaging.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Beata
      foaf_name: Turoňová, Beata
      foaf_surname: Turoňová
  - foaf_Person:
      foaf_givenName: Florian
      foaf_name: Schur, Florian
      foaf_surname: Schur
      foaf_workInfoHomepage: http://www.librecat.org/personId=48AD8942-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-4790-8078
  - foaf_Person:
      foaf_givenName: William
      foaf_name: Wan, William
      foaf_surname: Wan
  - foaf_Person:
      foaf_givenName: John
      foaf_name: Briggs, John
      foaf_surname: Briggs
  bibo_doi: 10.1016/j.jsb.2017.07.007
  bibo_issue: '3'
  bibo_volume: 199
  dct_date: 2017^xs_gYear
  dct_language: eng
  dct_publisher: Academic Press@
  dct_title: Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF
    improves subtomogram averaging resolution to 3.4Å@
...