[{"oa":1,"quality_controlled":"1","date_created":"2020-08-10T11:54:03Z","date_published":"2016-06-30T00:00:00Z","uri_base":"https://research-explorer.ista.ac.at","publication":"OncoImmunology","dc":{"creator":["Singer, Josef","Manzano-Szalai, Krisztina","Fazekas, Judit","Thell, Kathrin","Bentley-Lukschal, Anna","Stremnitzer, Caroline","Roth-Walter, Franziska","Weghofer, Margit","Ritter, Mirko","Pino Tossi, Kerstin","Hörer, Markus","Michaelis, Uwe","Jensen-Jarolim, Erika"],"language":["eng"],"rights":["info:eu-repo/semantics/openAccess"],"title":["Proof of concept study with an HER-2 mimotope anticancer vaccine deduced from a novel AAV-mimotope library platform"],"source":["Singer J, Manzano-Szalai K, Singer J, et al. Proof of concept study with an HER-2 mimotope anticancer vaccine deduced from a novel AAV-mimotope library platform. OncoImmunology. 2016;5(7). doi:10.1080/2162402x.2016.1171446"],"relation":["info:eu-repo/semantics/altIdentifier/doi/10.1080/2162402x.2016.1171446","info:eu-repo/semantics/altIdentifier/issn/2162-402X"],"description":["Background: Anticancer vaccines could represent a valuable complementary strategy to established therapies, especially in settings of early stage and minimal residual disease. HER-2 is an important target for immunotherapy and addressed by the monoclonal antibody trastuzumab. We have previously generated HER-2 mimotope peptides from phage display libraries. The synthesized peptides were coupled to carriers and applied for epitope-specific induction of trastuzumab-like IgG. For simplification and to avoid methodological limitations of synthesis and coupling chemistry, we herewith present a novel and optimized approach by using adeno-associated viruses (AAV) as effective and high-density mimotope-display system, which can be directly used for vaccination. Methods: An AAV capsid display library was constructed by genetically incorporating random peptides in a plasmid encoding the wild-type AAV2 capsid protein. AAV clones, expressing peptides specifically reactive to trastuzumab, were employed to immunize BALB/c mice. Antibody titers against human HER-2 were determined, and the isotype composition and functional properties of these were tested. Finally, prophylactically immunized mice were challenged with human HER-2 transfected mouse D2F2/E2 cells. Results: HER-2 mimotope AAV-vaccines induced antibodies specific to human HER-2. Two clones were selected for immunization of mice, which were subsequently grafted D2F2/E2 cells. Both mimotope AAV clones delayed the growth of tumors significantly, as compared to controls. Conclusion: In this study, a novel mimotope AAV-based platform was created allowing the isolation of mimotopes, which can be directly used as anticancer vaccines. The example of trastuzumab AAV-mimotopes demonstrates that this vaccine strategy could help to establish active immunotherapy for breast-cancer patients."],"identifier":["https://research-explorer.ista.ac.at/record/8241"],"date":["2016"],"type":["info:eu-repo/semantics/article","doc-type:article","text","http://purl.org/coar/resource_type/c_6501"],"publisher":["Taylor & Francis"]},"day":"30","article_number":"e1171446","article_processing_charge":"No","author":[{"first_name":"Josef","last_name":"Singer"},{"last_name":"Manzano-Szalai","first_name":"Krisztina"},{"first_name":"Judit","id":"36432834-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8777-3502","last_name":"Fazekas"},{"last_name":"Thell","first_name":"Kathrin"},{"last_name":"Bentley-Lukschal","first_name":"Anna"},{"last_name":"Stremnitzer","first_name":"Caroline"},{"first_name":"Franziska","last_name":"Roth-Walter"},{"first_name":"Margit","last_name":"Weghofer"},{"last_name":"Ritter","first_name":"Mirko"},{"first_name":"Kerstin","last_name":"Pino Tossi"},{"first_name":"Markus","last_name":"Hörer"},{"first_name":"Uwe","last_name":"Michaelis"},{"last_name":"Jensen-Jarolim","first_name":"Erika"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Singer J, Manzano-Szalai K, Singer J, Thell K, Bentley-Lukschal A, Stremnitzer C, Roth-Walter F, Weghofer M, Ritter M, Pino Tossi K, Hörer M, Michaelis U, Jensen-Jarolim E. 2016. Proof of concept study with an HER-2 mimotope anticancer vaccine deduced from a novel AAV-mimotope library platform. OncoImmunology. 5(7), e1171446.","chicago":"Singer, Josef, Krisztina Manzano-Szalai, Judit Singer, Kathrin Thell, Anna Bentley-Lukschal, Caroline Stremnitzer, Franziska Roth-Walter, et al. “Proof of Concept Study with an HER-2 Mimotope Anticancer Vaccine Deduced from a Novel AAV-Mimotope Library Platform.” OncoImmunology. Taylor & Francis, 2016. https://doi.org/10.1080/2162402x.2016.1171446.","apa":"Singer, J., Manzano-Szalai, K., Singer, J., Thell, K., Bentley-Lukschal, A., Stremnitzer, C., … Jensen-Jarolim, E. (2016). Proof of concept study with an HER-2 mimotope anticancer vaccine deduced from a novel AAV-mimotope library platform. OncoImmunology. Taylor & Francis. https://doi.org/10.1080/2162402x.2016.1171446","ieee":"J. Singer et al., “Proof of concept study with an HER-2 mimotope anticancer vaccine deduced from a novel AAV-mimotope library platform,” OncoImmunology, vol. 5, no. 7. Taylor & Francis, 2016.","short":"J. Singer, K. Manzano-Szalai, J. Singer, K. Thell, A. Bentley-Lukschal, C. Stremnitzer, F. Roth-Walter, M. Weghofer, M. Ritter, K. Pino Tossi, M. Hörer, U. Michaelis, E. Jensen-Jarolim, OncoImmunology 5 (2016).","mla":"Singer, Josef, et al. “Proof of Concept Study with an HER-2 Mimotope Anticancer Vaccine Deduced from a Novel AAV-Mimotope Library Platform.” OncoImmunology, vol. 5, no. 7, e1171446, Taylor & Francis, 2016, doi:10.1080/2162402x.2016.1171446."},"dini_type":"doc-type:article","intvolume":" 5","month":"06","main_file_link":[{"url":"https://doi.org/10.1080/2162402X.2016.1171446","open_access":"1"}],"oa_version":"Published Version","abstract":[{"lang":"eng"}],"volume":5,"issue":"7","language":[{}],"publication_status":"published","publication_identifier":{"issn":[]},"status":"public","article_type":"original","type":"journal_article","_id":"8241","extern":"1","creator":{"login":"jsinger","id":"36432834-F248-11E8-B48F-1D18A9856A87"},"date_updated":"2021-01-12T08:17:41Z"}]