---
_id: '831'
abstract:
- lang: eng
text: In Arabidopsis, lateral roots originate from pericycle cells deep within the
primary root. New lateral root primordia (LRP) have to emerge through several
overlaying tissues. Here, we report that auxin produced in new LRP is transported
towards the outer tissues where it triggers cell separation by inducing both the
auxin influx carrier LAX3 and cell-wall enzymes. LAX3 is expressed in just two
cell files overlaying new LRP. To understand how this striking pattern of LAX3
expression is regulated, we developed a mathematical model that captures the network
regulating its expression and auxin transport within realistic three-dimensional
cell and tissue geometries. Our model revealed that, for the LAX3 spatial expression
to be robust to natural variations in root tissue geometry, an efflux carrier
is required--later identified to be PIN3. To prevent LAX3 from being transiently
expressed in multiple cell files, PIN3 and LAX3 must be induced consecutively,
which we later demonstrated to be the case. Our study exemplifies how mathematical
models can be used to direct experiments to elucidate complex developmental processes.
acknowledgement: This work was supported by an FEBS Long‐Term Fellowship (BP), an
Intra‐European Fellowship for Career Development under the 7th framework of the
European Commission (IEF‐2008‐220506 to BP), an EMBO Long‐Term Fellowship (BP),
an European Reintegration Grant under the 7th framework of the European Commission
(ERG‐2010‐276662 to BP) and the Swedish Research Council (VR 621‐2010‐5720 to IS,
GS and KL). AMM, APF, AL, LRB, SP, NM, DMW, MO, JRK and MJB acknowledge the support
of the Biotechnology and Biological Sciences Research Council (BBSRC) and Engineering
and Physical Sciences Research Council (EPSRC) funding to the Centre for Plant Integrative
Biology (CPIB); BBSRC Professorial Research Fellowship funding to DMW and MJB; Belgian
Scientific policy (BELSPO contract MARS) to TB and MJB. We thank Bert de Rybel for
his help in Multisite Gateway cloning.
author:
- first_name: Benjamin
full_name: Péret, Benjamin
last_name: Péret
- first_name: Alistair
full_name: Middleton, Alistair M
last_name: Middleton
- first_name: Andrew
full_name: French, Andrew P
last_name: French
- first_name: Antoine
full_name: Larrieu, Antoine
last_name: Larrieu
- first_name: Anthony
full_name: Bishopp, Anthony
last_name: Bishopp
- first_name: Maria
full_name: Njo, Maria
last_name: Njo
- first_name: Darren
full_name: Wells, Darren M
last_name: Wells
- first_name: Silvana
full_name: Porco, Silvana
last_name: Porco
- first_name: Nathan
full_name: Mellor, Nathan
last_name: Mellor
- first_name: Leah
full_name: Band, Leah R
last_name: Band
- first_name: Ilda
full_name: Casimiro, Ilda
last_name: Casimiro
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Ilkka
full_name: Sairanen, Ilkka
last_name: Sairanen
- first_name: Romain
full_name: Mallet, Romain
last_name: Mallet
- first_name: Göran
full_name: Sandberg, Göran
last_name: Sandberg
- first_name: Karin
full_name: Ljung, Karin
last_name: Ljung
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eric
full_name: Kramer, Eric
last_name: Kramer
- first_name: John
full_name: King, John R
last_name: King
- first_name: Ive
full_name: De Smet, Ive
last_name: De Smet
- first_name: Tony
full_name: Pridmore, Tony
last_name: Pridmore
- first_name: Markus
full_name: Owen, Markus
last_name: Owen
- first_name: Malcolm
full_name: Bennett, Malcolm J
last_name: Bennett
citation:
ama: Péret B, Middleton A, French A, et al. Sequential induction of auxin efflux
and influx carriers regulates lateral root emergence. Molecular Systems Biology.
2013;9. doi:10.1038/msb.2013.43
apa: Péret, B., Middleton, A., French, A., Larrieu, A., Bishopp, A., Njo, M., …
Bennett, M. (2013). Sequential induction of auxin efflux and influx carriers regulates
lateral root emergence. Molecular Systems Biology. Nature Publishing Group.
https://doi.org/10.1038/msb.2013.43
chicago: Péret, Benjamin, Alistair Middleton, Andrew French, Antoine Larrieu, Anthony
Bishopp, Maria Njo, Darren Wells, et al. “Sequential Induction of Auxin Efflux
and Influx Carriers Regulates Lateral Root Emergence.” Molecular Systems Biology.
Nature Publishing Group, 2013. https://doi.org/10.1038/msb.2013.43.
ieee: B. Péret et al., “Sequential induction of auxin efflux and influx carriers
regulates lateral root emergence,” Molecular Systems Biology, vol. 9. Nature
Publishing Group, 2013.
ista: Péret B, Middleton A, French A, Larrieu A, Bishopp A, Njo M, Wells D, Porco
S, Mellor N, Band L, Casimiro I, Kleine Vehn J, Vanneste S, Sairanen I, Mallet
R, Sandberg G, Ljung K, Beeckman T, Benková E, Friml J, Kramer E, King J, De Smet
I, Pridmore T, Owen M, Bennett M. 2013. Sequential induction of auxin efflux and
influx carriers regulates lateral root emergence. Molecular Systems Biology. 9.
mla: Péret, Benjamin, et al. “Sequential Induction of Auxin Efflux and Influx Carriers
Regulates Lateral Root Emergence.” Molecular Systems Biology, vol. 9, Nature
Publishing Group, 2013, doi:10.1038/msb.2013.43.
short: B. Péret, A. Middleton, A. French, A. Larrieu, A. Bishopp, M. Njo, D. Wells,
S. Porco, N. Mellor, L. Band, I. Casimiro, J. Kleine Vehn, S. Vanneste, I. Sairanen,
R. Mallet, G. Sandberg, K. Ljung, T. Beeckman, E. Benková, J. Friml, E. Kramer,
J. King, I. De Smet, T. Pridmore, M. Owen, M. Bennett, Molecular Systems Biology
9 (2013).
date_created: 2018-12-11T11:48:44Z
date_published: 2013-10-22T00:00:00Z
date_updated: 2021-01-12T08:18:03Z
day: '22'
doi: 10.1038/msb.2013.43
extern: 1
intvolume: ' 9'
month: '10'
publication: Molecular Systems Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '6817'
quality_controlled: 0
status: public
title: Sequential induction of auxin efflux and influx carriers regulates lateral
root emergence
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: journal_article
volume: 9
year: '2013'
...