---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '832'
abstract:
- lang: eng
  text: Plants maintain capacity to form new organs such as leaves, flowers, lateral
    shoots and roots throughout their postembryonic lifetime. Lateral roots (LRs)
    originate from a few pericycle cells that acquire attributes of founder cells
    (FCs), undergo series of anticlinal divisions, and give rise to a few short initial
    cells. After initiation, coordinated cell division and differentiation occur,
    giving rise to lateral root primordia (LRP). Primordia continue to grow, emerge
    through the cortex and epidermal layers of the primary root, and finally a new
    apical meristem is established taking over the responsibility for growth of mature
    lateral roots [for detailed description of the individual stages of lateral root
    organogenesis see Malamy and Benfey (1997)]. To examine this highly dynamic developmental
    process and to investigate a role of various hormonal, genetic and environmental
    factors in the regulation of lateral root organogenesis, the real time imaging
    based analyses represent extremely powerful tools (Laskowski et al., 2008; De
    Smet et al., 2012; Marhavy et al., 2013 and 2014). Herein, we describe a protocol
    for real time lateral root primordia (LRP) analysis, which enables the monitoring
    of an onset of the specific gene expression and subcellular protein localization
    during primordia organogenesis, as well as the evaluation of the impact of genetic
    and environmental perturbations on LRP organogenesis.
acknowledgement: "European Research Council with a Starting Independent Research grant:
  ERC-2007-Stg-207362-HCPO, Czech Science Foundation: GA13-39982S\r\nWe thank Matyas
  Fendrych for critical reading and comments. The protocol was developed based on
  previously published work of De Rybel et al. (2010) and Laskowski et al. (2008). "
article_processing_charge: No
article_type: original
author:
- first_name: Peter
  full_name: Marhavy, Peter
  id: 3F45B078-F248-11E8-B48F-1D18A9856A87
  last_name: Marhavy
  orcid: 0000-0001-5227-5741
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
citation:
  ama: Marhavý P, Benková E. Real time analysis of lateral root organogenesis in arabidopsis.
    <i>Bio-protocol</i>. 2015;5(8). doi:<a href="https://doi.org/10.21769/BioProtoc.1446">10.21769/BioProtoc.1446</a>
  apa: Marhavý, P., &#38; Benková, E. (2015). Real time analysis of lateral root organogenesis
    in arabidopsis. <i>Bio-Protocol</i>. Bio-protocol LLC. <a href="https://doi.org/10.21769/BioProtoc.1446">https://doi.org/10.21769/BioProtoc.1446</a>
  chicago: Marhavý, Peter, and Eva Benková. “Real Time Analysis of Lateral Root Organogenesis
    in Arabidopsis.” <i>Bio-Protocol</i>. Bio-protocol LLC, 2015. <a href="https://doi.org/10.21769/BioProtoc.1446">https://doi.org/10.21769/BioProtoc.1446</a>.
  ieee: P. Marhavý and E. Benková, “Real time analysis of lateral root organogenesis
    in arabidopsis,” <i>Bio-protocol</i>, vol. 5, no. 8. Bio-protocol LLC, 2015.
  ista: Marhavý P, Benková E. 2015. Real time analysis of lateral root organogenesis
    in arabidopsis. Bio-protocol. 5(8).
  mla: Marhavý, Peter, and Eva Benková. “Real Time Analysis of Lateral Root Organogenesis
    in Arabidopsis.” <i>Bio-Protocol</i>, vol. 5, no. 8, Bio-protocol LLC, 2015, doi:<a
    href="https://doi.org/10.21769/BioProtoc.1446">10.21769/BioProtoc.1446</a>.
  short: P. Marhavý, E. Benková, Bio-Protocol 5 (2015).
date_created: 2018-12-11T11:48:44Z
date_published: 2015-04-20T00:00:00Z
date_updated: 2026-05-19T09:39:29Z
day: '20'
doi: 10.21769/BioProtoc.1446
extern: '1'
external_id:
  pmid:
  - '27331080'
intvolume: '         5'
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.21769/BioProtoc.1446
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Bio-protocol
publication_identifier:
  eissn:
  - 2331-8325
publication_status: published
publisher: Bio-protocol LLC
publist_id: '6816'
quality_controlled: '1'
status: public
title: Real time analysis of lateral root organogenesis in arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 5
year: '2015'
...