Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum Kubiasova, Karolina ; https://orcid.org/0000-0001-5630-9419 Montesinos López, Juan C ; https://orcid.org/0000-0001-9179-6099 Šamajová, Olga Nisler, Jaroslav Mik, Václav Semeradova, Hana Plíhalová, Lucie Novák, Ondřej Marhavý, Peter ; https://orcid.org/0000-0001-5227-5741 Cavallari, Nicola Zalabák, David Berka, Karel Doležal, Karel Galuszka, Petr Šamaj, Jozef Strnad, Miroslav Benková, Eva ; https://orcid.org/0000-0002-8510-9739 Plíhal, Ondřej Spíchal, Lukáš ddc:580 Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER. Springer Nature 2020 info:eu-repo/semantics/article doc-type:Article Article http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/8336 https://research-explorer.ista.ac.at/download/8336/8357 Kubiasova K, Montesinos López JC, Šamajová O, et al. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. <i>Nature Communications</i>. 2020;11. doi:<a href="https://doi.org/10.1038/s41467-020-17949-0">10.1038/s41467-020-17949-0</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-020-17949-0 info:eu-repo/semantics/altIdentifier/e-issn/2041-1723 info:eu-repo/semantics/altIdentifier/wos/000567931000002 info:eu-repo/semantics/altIdentifier/pmid/32855390 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess