Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum

article Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum published yes Karolina Kubiasova author 946011F4-3E71-11EA-860B-C7A73DDC885E0000-0001-5630-9419 Juan C Montesinos López author 310A8E3E-F248-11E8-B48F-1D18A9856A870000-0001-9179-6099 Olga Šamajová author Jaroslav Nisler author Václav Mik author Hana Semeradova author 42FE702E-F248-11E8-B48F-1D18A9856A87 Lucie Plíhalová author Ondřej Novák author Peter Marhavý author 3F45B078-F248-11E8-B48F-1D18A9856A870000-0001-5227-5741 Nicola Cavallari author 457160E6-F248-11E8-B48F-1D18A9856A87 David Zalabák author Karel Berka author Karel Doležal author Petr Galuszka author Jozef Šamaj author Miroslav Strnad author Eva Benková author 38F4F166-F248-11E8-B48F-1D18A9856A870000-0002-8510-9739 Ondřej Plíhal author Lukáš Spíchal author EvBe department International IST Postdoc Fellowship Programme project Molecular mechanisms of the cytokinin regulated endomembrane trafficking to coordinate plant organogenesis project Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants project Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER. https://research-explorer.ista.ac.at/download/8336/8357/2020_NatureComm_Kubiasova.pdf application/pdfno https://creativecommons.org/licenses/by/4.0/ Springer Nature2020 eng Nature Communications 2041-1723 32855390 00056793100000210.1038/s41467-020-17949-0 11 Kubiasova, Karolina, et al. “Cytokinin Fluoroprobe Reveals Multiple Sites of Cytokinin Perception at Plasma Membrane and Endoplasmic Reticulum.” Nature Communications, vol. 11, 4285, Springer Nature, 2020, doi:<a href="https://doi.org/10.1038/s41467-020-17949-0">10.1038/s41467-020-17949-0</a>. Kubiasova, Karolina, Juan C Montesinos López, Olga Šamajová, Jaroslav Nisler, Václav Mik, Hana Semerádová, Lucie Plíhalová, et al. “Cytokinin Fluoroprobe Reveals Multiple Sites of Cytokinin Perception at Plasma Membrane and Endoplasmic Reticulum.” Nature Communications. Springer Nature, 2020. <a href="https://doi.org/10.1038/s41467-020-17949-0">https://doi.org/10.1038/s41467-020-17949-0</a>. K. Kubiasova et al., “Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum,” Nature Communications, vol. 11. Springer Nature, 2020. Kubiasova, K., Montesinos López, J. C., Šamajová, O., Nisler, J., Mik, V., Semerádová, H., … Spíchal, L. (2020). Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. Springer Nature. <a href="https://doi.org/10.1038/s41467-020-17949-0">https://doi.org/10.1038/s41467-020-17949-0</a> Kubiasova K, Montesinos López JC, Šamajová O, Nisler J, Mik V, Semerádová H, Plíhalová L, Novák O, Marhavý P, Cavallari N, Zalabák D, Berka K, Doležal K, Galuszka P, Šamaj J, Strnad M, Benková E, Plíhal O, Spíchal L. 2020. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. 11, 4285. K. Kubiasova, J.C. Montesinos López, O. Šamajová, J. Nisler, V. Mik, H. Semerádová, L. Plíhalová, O. Novák, P. Marhavý, N. Cavallari, D. Zalabák, K. Berka, K. Doležal, P. Galuszka, J. Šamaj, M. Strnad, E. Benková, O. Plíhal, L. Spíchal, Nature Communications 11 (2020). Kubiasova K, Montesinos López JC, Šamajová O, et al. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. 2020;11. doi:<a href="https://doi.org/10.1038/s41467-020-17949-0">10.1038/s41467-020-17949-0</a> 83362020-09-06T22:01:12Z2026-04-02T14:35:13Z