--- res: bibo_abstract: - Biophysical investigation of membrane proteins generally requires their extraction from native sources using detergents, a step that can lead, possibly irreversibly, to protein denaturation. The propensity of dodecylphosphocholine (DPC), a detergent widely utilized in NMR studies of membrane proteins, to distort their structure has been the subject of much controversy. It has been recently proposed that the binding specificity of the yeast mitochondrial ADP/ATP carrier (yAAC3) toward cardiolipins is preserved in DPC, thereby suggesting that DPC is a suitable environment in which to study membrane proteins. In this communication, we used all-atom molecular dynamics simulations to investigate the specific binding of cardiolipins to yAAC3. Our data demonstrate that the interaction interface observed in a native-like environment differs markedly from that inferred from an NMR investigation in DPC, implying that in this detergent, the protein structure is distorted. We further investigated yAAC3 solubilized in DPC and in the milder dodecylmaltoside with thermal-shift assays. The loss of thermal transition observed in DPC confirms that the protein is no longer properly folded in this environment.@eng bibo_authorlist: - foaf_Person: foaf_givenName: François foaf_name: Dehez, François foaf_surname: Dehez - foaf_Person: foaf_givenName: Paul foaf_name: Schanda, Paul foaf_surname: Schanda foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425 orcid: 0000-0002-9350-7606 - foaf_Person: foaf_givenName: Martin S. foaf_name: King, Martin S. foaf_surname: King - foaf_Person: foaf_givenName: Edmund R.S. foaf_name: Kunji, Edmund R.S. foaf_surname: Kunji - foaf_Person: foaf_givenName: Christophe foaf_name: Chipot, Christophe foaf_surname: Chipot bibo_doi: 10.1016/j.bpj.2017.09.019 bibo_issue: '11' bibo_volume: 113 dct_date: 2017^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/0006-3495 dct_language: eng dct_publisher: Elsevier@ dct_subject: - Biophysics dct_title: Mitochondrial ADP/ATP carrier in dodecylphosphocholine binds cardiolipins with non-native affinity@ ...