--- res: bibo_abstract: - Ensuring the correct folding of RNA molecules in the cell is of major importance for a large variety of biological functions. Therefore, chaperone proteins that assist RNA in adopting their functionally active states are abundant in all living organisms. An important feature of RNA chaperone proteins is that they do not require an external energy source to perform their activity, and that they interact transiently and non-specifically with their RNA targets. So far, little is known about the mechanistic details of the RNA chaperone activity of these proteins. Prominent examples of RNA chaperones are bacterial cold shock proteins (Csp) that have been reported to bind single-stranded RNA and DNA. Here, we have used advanced NMR spectroscopy techniques to investigate at atomic resolution the RNA-melting activity of CspA, the major cold shock protein of Escherichia coli, upon binding to different RNA hairpins. Real-time NMR provides detailed information on the folding kinetics and folding pathways. Finally, comparison of wild-type CspA with single-point mutants and small peptides yields insights into the complementary roles of aromatic and positively charged amino-acid side chains for the RNA chaperone activity of the protein.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Enrico foaf_name: Rennella, Enrico foaf_surname: Rennella - foaf_Person: foaf_givenName: Tomáš foaf_name: Sára, Tomáš foaf_surname: Sára - foaf_Person: foaf_givenName: Michael foaf_name: Juen, Michael foaf_surname: Juen - foaf_Person: foaf_givenName: Christoph foaf_name: Wunderlich, Christoph foaf_surname: Wunderlich - foaf_Person: foaf_givenName: Lionel foaf_name: Imbert, Lionel foaf_surname: Imbert - foaf_Person: foaf_givenName: Zsofia foaf_name: Solyom, Zsofia foaf_surname: Solyom - foaf_Person: foaf_givenName: Adrien foaf_name: Favier, Adrien foaf_surname: Favier - foaf_Person: foaf_givenName: Isabel foaf_name: Ayala, Isabel foaf_surname: Ayala - foaf_Person: foaf_givenName: Katharina foaf_name: Weinhäupl, Katharina foaf_surname: Weinhäupl - foaf_Person: foaf_givenName: Paul foaf_name: Schanda, Paul foaf_surname: Schanda foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425 orcid: 0000-0002-9350-7606 - foaf_Person: foaf_givenName: Robert foaf_name: Konrat, Robert foaf_surname: Konrat - foaf_Person: foaf_givenName: Christoph foaf_name: Kreutz, Christoph foaf_surname: Kreutz - foaf_Person: foaf_givenName: Bernhard foaf_name: Brutscher, Bernhard foaf_surname: Brutscher bibo_doi: 10.1093/nar/gkx044 bibo_issue: '7' bibo_volume: 45 dct_date: 2017^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/0305-1048 - http://id.crossref.org/issn/1362-4962 dct_language: eng dct_publisher: Oxford University Press@ dct_title: RNA binding and chaperone activity of the E.coli cold-shock protein CspA@ ...