A set of BEST triple-resonance experiments for time-optimized protein resonance assignment Lescop, Ewen Schanda, Paul ; https://orcid.org/0000-0002-9350-7606 Brutscher, Bernhard A series of sequential, intra-residue, and bi-directional BEST H–N–CA, H–N–CO, and H–N–CB pulse sequences is presented that extends the BEST concept introduced recently for fast multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the complete set of experiments required for sequential resonance assignment. We demonstrate for the protein ubiquitin that 3D BEST H–N–C correlation spectra can be recorded on a 600 MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of acquisition time with sufficient sensitivity to detect all expected cross peaks. Elsevier 2007 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_6501 https://research-explorer.ista.ac.at/record/8484 Lescop E, Schanda P, Brutscher B. A set of BEST triple-resonance experiments for time-optimized protein resonance assignment. <i>Journal of Magnetic Resonance</i>. 2007;187(1):163-169. doi:<a href="https://doi.org/10.1016/j.jmr.2007.04.002">10.1016/j.jmr.2007.04.002</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jmr.2007.04.002 info:eu-repo/semantics/altIdentifier/issn/1090-7807 info:eu-repo/semantics/closedAccess