article published yes Ewen Lescop author Paul Schanda author 7B541462-FAF6-11E9-A490-E8DFE56974250000-0002-9350-7606 Bernhard Brutscher author A series of sequential, intra-residue, and bi-directional BEST H–N–CA, H–N–CO, and H–N–CB pulse sequences is presented that extends the BEST concept introduced recently for fast multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the complete set of experiments required for sequential resonance assignment. We demonstrate for the protein ubiquitin that 3D BEST H–N–C correlation spectra can be recorded on a 600 MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of acquisition time with sufficient sensitivity to detect all expected cross peaks. Elsevier2007 eng 1090-780710.1016/j.jmr.2007.04.002 1871163-169 yes Lescop, E., Schanda, P., &#38; Brutscher, B. (2007). A set of BEST triple-resonance experiments for time-optimized protein resonance assignment. <i>Journal of Magnetic Resonance</i>. Elsevier. <a href="https://doi.org/10.1016/j.jmr.2007.04.002">https://doi.org/10.1016/j.jmr.2007.04.002</a> Lescop E, Schanda P, Brutscher B. A set of BEST triple-resonance experiments for time-optimized protein resonance assignment. <i>Journal of Magnetic Resonance</i>. 2007;187(1):163-169. doi:<a href="https://doi.org/10.1016/j.jmr.2007.04.002">10.1016/j.jmr.2007.04.002</a> Lescop, Ewen, Paul Schanda, and Bernhard Brutscher. “A Set of BEST Triple-Resonance Experiments for Time-Optimized Protein Resonance Assignment.” <i>Journal of Magnetic Resonance</i>. Elsevier, 2007. <a href="https://doi.org/10.1016/j.jmr.2007.04.002">https://doi.org/10.1016/j.jmr.2007.04.002</a>. E. Lescop, P. Schanda, and B. Brutscher, “A set of BEST triple-resonance experiments for time-optimized protein resonance assignment,” <i>Journal of Magnetic Resonance</i>, vol. 187, no. 1. Elsevier, pp. 163–169, 2007. Lescop, Ewen, et al. “A Set of BEST Triple-Resonance Experiments for Time-Optimized Protein Resonance Assignment.” <i>Journal of Magnetic Resonance</i>, vol. 187, no. 1, Elsevier, 2007, pp. 163–69, doi:<a href="https://doi.org/10.1016/j.jmr.2007.04.002">10.1016/j.jmr.2007.04.002</a>. E. Lescop, P. Schanda, B. Brutscher, Journal of Magnetic Resonance 187 (2007) 163–169. Lescop E, Schanda P, Brutscher B. 2007. A set of BEST triple-resonance experiments for time-optimized protein resonance assignment. Journal of Magnetic Resonance. 187(1), 163–169. 84842020-09-18T10:13:02Z2021-01-12T08:19:35Z