---
_id: '8485'
abstract:
- lang: eng
  text: High signal to noise is a necessity for the quantification of NMR spectral
    parameters to be translated into accurate and precise restraints on protein structure
    and dynamics. An important source of long-range structural information is obtained
    from 1H–1H residual dipolar couplings (RDCs) measured for weakly aligned molecules.
    For sensitivity reasons, such measurements are generally performed on highly deuterated
    protein samples. Here we show that high sensitivity is also obtained for protonated
    protein samples if the pulse schemes are optimized in terms of longitudinal relaxation
    efficiency and J-mismatch compensated coherence transfer. The new sensitivity-optimized
    quantitative J-correlation experiment yields important signal gains reaching factors
    of 1.5 to 8 for individual correlation peaks when compared to previously proposed
    pulse schemes.
article_processing_charge: No
article_type: original
author:
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Ewen
  full_name: Lescop, Ewen
  last_name: Lescop
- first_name: Mirjam
  full_name: Falge, Mirjam
  last_name: Falge
- first_name: Rémy
  full_name: Sounier, Rémy
  last_name: Sounier
- first_name: Jérôme
  full_name: Boisbouvier, Jérôme
  last_name: Boisbouvier
- first_name: Bernhard
  full_name: Brutscher, Bernhard
  last_name: Brutscher
citation:
  ama: Schanda P, Lescop E, Falge M, Sounier R, Boisbouvier J, Brutscher B. Sensitivity-optimized
    experiment for the measurement of residual dipolar couplings between amide protons.
    <i>Journal of Biomolecular NMR</i>. 2007;38:47-55. doi:<a href="https://doi.org/10.1007/s10858-006-9138-2">10.1007/s10858-006-9138-2</a>
  apa: Schanda, P., Lescop, E., Falge, M., Sounier, R., Boisbouvier, J., &#38; Brutscher,
    B. (2007). Sensitivity-optimized experiment for the measurement of residual dipolar
    couplings between amide protons. <i>Journal of Biomolecular NMR</i>. Springer
    Nature. <a href="https://doi.org/10.1007/s10858-006-9138-2">https://doi.org/10.1007/s10858-006-9138-2</a>
  chicago: Schanda, Paul, Ewen Lescop, Mirjam Falge, Rémy Sounier, Jérôme Boisbouvier,
    and Bernhard Brutscher. “Sensitivity-Optimized Experiment for the Measurement
    of Residual Dipolar Couplings between Amide Protons.” <i>Journal of Biomolecular
    NMR</i>. Springer Nature, 2007. <a href="https://doi.org/10.1007/s10858-006-9138-2">https://doi.org/10.1007/s10858-006-9138-2</a>.
  ieee: P. Schanda, E. Lescop, M. Falge, R. Sounier, J. Boisbouvier, and B. Brutscher,
    “Sensitivity-optimized experiment for the measurement of residual dipolar couplings
    between amide protons,” <i>Journal of Biomolecular NMR</i>, vol. 38. Springer
    Nature, pp. 47–55, 2007.
  ista: Schanda P, Lescop E, Falge M, Sounier R, Boisbouvier J, Brutscher B. 2007.
    Sensitivity-optimized experiment for the measurement of residual dipolar couplings
    between amide protons. Journal of Biomolecular NMR. 38, 47–55.
  mla: Schanda, Paul, et al. “Sensitivity-Optimized Experiment for the Measurement
    of Residual Dipolar Couplings between Amide Protons.” <i>Journal of Biomolecular
    NMR</i>, vol. 38, Springer Nature, 2007, pp. 47–55, doi:<a href="https://doi.org/10.1007/s10858-006-9138-2">10.1007/s10858-006-9138-2</a>.
  short: P. Schanda, E. Lescop, M. Falge, R. Sounier, J. Boisbouvier, B. Brutscher,
    Journal of Biomolecular NMR 38 (2007) 47–55.
date_created: 2020-09-18T10:13:12Z
date_published: 2007-03-08T00:00:00Z
date_updated: 2021-01-12T08:19:36Z
day: '08'
doi: 10.1007/s10858-006-9138-2
extern: '1'
intvolume: '        38'
keyword:
- Spectroscopy
- Biochemistry
language:
- iso: eng
month: '03'
oa_version: None
page: 47-55
publication: Journal of Biomolecular NMR
publication_identifier:
  issn:
  - 0925-2738
  - 1573-5001
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: Sensitivity-optimized experiment for the measurement of residual dipolar couplings
  between amide protons
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 38
year: '2007'
...