---
_id: '8489'
abstract:
- lang: eng
text: Structure elucidation of proteins by either NMR or X‐ray crystallography often
requires the screening of a large number of samples for promising protein constructs
and optimum solution conditions. For large‐scale screening of protein samples
in solution, robust methods are needed that allow a rapid assessment of the folding
of a polypeptide under diverse sample conditions. Here we present HET‐SOFAST NMR,
a highly sensitive new method for semi‐quantitative characterization of the structural
compactness and heterogeneity of polypeptide chains in solution. On the basis
of one‐dimensional 1H HET‐SOFAST NMR data, obtained on well‐folded, molten globular,
partially‐ and completely unfolded proteins, we define empirical thresholds that
can be used as quantitative benchmarks for protein compactness. For 15N‐enriched
protein samples, two‐dimensional 1H‐15N HET‐SOFAST correlation spectra provide
site‐specific information about the structural heterogeneity along the polypeptide
chain.
article_processing_charge: No
article_type: original
author:
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
- first_name: Vincent
full_name: Forge, Vincent
last_name: Forge
- first_name: Bernhard
full_name: Brutscher, Bernhard
last_name: Brutscher
citation:
ama: Schanda P, Forge V, Brutscher B. HET-SOFAST NMR for fast detection of structural
compactness and heterogeneity along polypeptide chains. Magnetic Resonance
in Chemistry. 2006;44(S1):S177-S184. doi:10.1002/mrc.1825
apa: Schanda, P., Forge, V., & Brutscher, B. (2006). HET-SOFAST NMR for fast
detection of structural compactness and heterogeneity along polypeptide chains.
Magnetic Resonance in Chemistry. Wiley. https://doi.org/10.1002/mrc.1825
chicago: Schanda, Paul, Vincent Forge, and Bernhard Brutscher. “HET-SOFAST NMR for
Fast Detection of Structural Compactness and Heterogeneity along Polypeptide Chains.”
Magnetic Resonance in Chemistry. Wiley, 2006. https://doi.org/10.1002/mrc.1825.
ieee: P. Schanda, V. Forge, and B. Brutscher, “HET-SOFAST NMR for fast detection
of structural compactness and heterogeneity along polypeptide chains,” Magnetic
Resonance in Chemistry, vol. 44, no. S1. Wiley, pp. S177–S184, 2006.
ista: Schanda P, Forge V, Brutscher B. 2006. HET-SOFAST NMR for fast detection of
structural compactness and heterogeneity along polypeptide chains. Magnetic Resonance
in Chemistry. 44(S1), S177–S184.
mla: Schanda, Paul, et al. “HET-SOFAST NMR for Fast Detection of Structural Compactness
and Heterogeneity along Polypeptide Chains.” Magnetic Resonance in Chemistry,
vol. 44, no. S1, Wiley, 2006, pp. S177–84, doi:10.1002/mrc.1825.
short: P. Schanda, V. Forge, B. Brutscher, Magnetic Resonance in Chemistry 44 (2006)
S177–S184.
date_created: 2020-09-18T10:13:42Z
date_published: 2006-07-06T00:00:00Z
date_updated: 2021-01-12T08:19:37Z
day: '06'
doi: 10.1002/mrc.1825
extern: '1'
intvolume: ' 44'
issue: S1
language:
- iso: eng
month: '07'
oa_version: None
page: S177-S184
publication: Magnetic Resonance in Chemistry
publication_identifier:
issn:
- 0749-1581
- 1097-458X
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: HET-SOFAST NMR for fast detection of structural compactness and heterogeneity
along polypeptide chains
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2006'
...