--- _id: '8489' abstract: - lang: eng text: Structure elucidation of proteins by either NMR or X‐ray crystallography often requires the screening of a large number of samples for promising protein constructs and optimum solution conditions. For large‐scale screening of protein samples in solution, robust methods are needed that allow a rapid assessment of the folding of a polypeptide under diverse sample conditions. Here we present HET‐SOFAST NMR, a highly sensitive new method for semi‐quantitative characterization of the structural compactness and heterogeneity of polypeptide chains in solution. On the basis of one‐dimensional 1H HET‐SOFAST NMR data, obtained on well‐folded, molten globular, partially‐ and completely unfolded proteins, we define empirical thresholds that can be used as quantitative benchmarks for protein compactness. For 15N‐enriched protein samples, two‐dimensional 1H‐15N HET‐SOFAST correlation spectra provide site‐specific information about the structural heterogeneity along the polypeptide chain. article_processing_charge: No article_type: original author: - first_name: Paul full_name: Schanda, Paul id: 7B541462-FAF6-11E9-A490-E8DFE5697425 last_name: Schanda orcid: 0000-0002-9350-7606 - first_name: Vincent full_name: Forge, Vincent last_name: Forge - first_name: Bernhard full_name: Brutscher, Bernhard last_name: Brutscher citation: ama: Schanda P, Forge V, Brutscher B. HET-SOFAST NMR for fast detection of structural compactness and heterogeneity along polypeptide chains. Magnetic Resonance in Chemistry. 2006;44(S1):S177-S184. doi:10.1002/mrc.1825 apa: Schanda, P., Forge, V., & Brutscher, B. (2006). HET-SOFAST NMR for fast detection of structural compactness and heterogeneity along polypeptide chains. Magnetic Resonance in Chemistry. Wiley. https://doi.org/10.1002/mrc.1825 chicago: Schanda, Paul, Vincent Forge, and Bernhard Brutscher. “HET-SOFAST NMR for Fast Detection of Structural Compactness and Heterogeneity along Polypeptide Chains.” Magnetic Resonance in Chemistry. Wiley, 2006. https://doi.org/10.1002/mrc.1825. ieee: P. Schanda, V. Forge, and B. Brutscher, “HET-SOFAST NMR for fast detection of structural compactness and heterogeneity along polypeptide chains,” Magnetic Resonance in Chemistry, vol. 44, no. S1. Wiley, pp. S177–S184, 2006. ista: Schanda P, Forge V, Brutscher B. 2006. HET-SOFAST NMR for fast detection of structural compactness and heterogeneity along polypeptide chains. Magnetic Resonance in Chemistry. 44(S1), S177–S184. mla: Schanda, Paul, et al. “HET-SOFAST NMR for Fast Detection of Structural Compactness and Heterogeneity along Polypeptide Chains.” Magnetic Resonance in Chemistry, vol. 44, no. S1, Wiley, 2006, pp. S177–84, doi:10.1002/mrc.1825. short: P. Schanda, V. Forge, B. Brutscher, Magnetic Resonance in Chemistry 44 (2006) S177–S184. date_created: 2020-09-18T10:13:42Z date_published: 2006-07-06T00:00:00Z date_updated: 2021-01-12T08:19:37Z day: '06' doi: 10.1002/mrc.1825 extern: '1' intvolume: ' 44' issue: S1 language: - iso: eng month: '07' oa_version: None page: S177-S184 publication: Magnetic Resonance in Chemistry publication_identifier: issn: - 0749-1581 - 1097-458X publication_status: published publisher: Wiley quality_controlled: '1' status: public title: HET-SOFAST NMR for fast detection of structural compactness and heterogeneity along polypeptide chains type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 44 year: '2006' ...