--- res: bibo_abstract: - Fast multidimensional NMR with a time resolution of a few seconds provides a new tool for high throughput screening and site-resolved real-time studies of kinetic molecular processes by NMR. Recently we have demonstrated the feasibility to record protein 1H–15N correlation spectra in a few seconds of acquisition time using a new SOFAST-HMQC experiment (Schanda and Brutscher (2005) J. Am. Chem. Soc. 127, 8014). Here, we investigate in detail the performance of SOFAST-HMQC to record 1H–15N and 1H−13C correlation spectra of proteins of different size and at different magnetic field strengths. Compared to standard 1H–15N correlation experiments SOFAST-HMQC provides a significant gain in sensitivity, especially for fast repetition rates. Guidelines are provided on how to set up SOFAST-HMQC experiments for a given protein sample. In addition, an alternative pulse scheme, IPAP-SOFAST-HMQC is presented that allows application on NMR spectrometers equipped with cryogenic probes, and fast measurement of one-bond 1H–13C and 1H–15N scalar and residual dipolar coupling constants.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Paul foaf_name: Schanda, Paul foaf_surname: Schanda foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425 orcid: 0000-0002-9350-7606 - foaf_Person: foaf_givenName: Ēriks foaf_name: Kupče, Ēriks foaf_surname: Kupče - foaf_Person: foaf_givenName: Bernhard foaf_name: Brutscher, Bernhard foaf_surname: Brutscher bibo_doi: 10.1007/s10858-005-4425-x bibo_issue: '4' bibo_volume: 33 dct_date: 2005^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/0925-2738 - http://id.crossref.org/issn/1573-5001 dct_language: eng dct_publisher: Springer Nature@ dct_subject: - Spectroscopy - Biochemistry dct_title: SOFAST-HMQC experiments for recording two-dimensional deteronuclear correlation spectra of proteins within a few seconds@ ...