SOFAST-HMQC experiments for recording two-dimensional deteronuclear correlation spectra of proteins within a few seconds Schanda, Paul ; https://orcid.org/0000-0002-9350-7606 Kupče, Ēriks Brutscher, Bernhard Spectroscopy Biochemistry Fast multidimensional NMR with a time resolution of a few seconds provides a new tool for high throughput screening and site-resolved real-time studies of kinetic molecular processes by NMR. Recently we have demonstrated the feasibility to record protein 1H–15N correlation spectra in a few seconds of acquisition time using a new SOFAST-HMQC experiment (Schanda and Brutscher (2005) J. Am. Chem. Soc. 127, 8014). Here, we investigate in detail the performance of SOFAST-HMQC to record 1H–15N and 1H−13C correlation spectra of proteins of different size and at different magnetic field strengths. Compared to standard 1H–15N correlation experiments SOFAST-HMQC provides a significant gain in sensitivity, especially for fast repetition rates. Guidelines are provided on how to set up SOFAST-HMQC experiments for a given protein sample. In addition, an alternative pulse scheme, IPAP-SOFAST-HMQC is presented that allows application on NMR spectrometers equipped with cryogenic probes, and fast measurement of one-bond 1H–13C and 1H–15N scalar and residual dipolar coupling constants. Springer Nature 2005 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/8491 Schanda P, Kupče Ē, Brutscher B. SOFAST-HMQC experiments for recording two-dimensional deteronuclear correlation spectra of proteins within a few seconds. <i>Journal of Biomolecular NMR</i>. 2005;33(4):199-211. doi:<a href="https://doi.org/10.1007/s10858-005-4425-x">10.1007/s10858-005-4425-x</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s10858-005-4425-x info:eu-repo/semantics/altIdentifier/issn/0925-2738 info:eu-repo/semantics/altIdentifier/issn/1573-5001 info:eu-repo/semantics/closedAccess