---
_id: '8491'
abstract:
- lang: eng
  text: Fast multidimensional NMR with a time resolution of a few seconds provides
    a new tool for high throughput screening and site-resolved real-time studies of
    kinetic molecular processes by NMR. Recently we have demonstrated the feasibility
    to record protein 1H–15N correlation spectra in a few seconds of acquisition time
    using a new SOFAST-HMQC experiment (Schanda and Brutscher (2005) J. Am. Chem.
    Soc. 127, 8014). Here, we investigate in detail the performance of SOFAST-HMQC
    to record 1H–15N and 1H−13C correlation spectra of proteins of different size
    and at different magnetic field strengths. Compared to standard 1H–15N correlation
    experiments SOFAST-HMQC provides a significant gain in sensitivity, especially
    for fast repetition rates. Guidelines are provided on how to set up SOFAST-HMQC
    experiments for a given protein sample. In addition, an alternative pulse scheme,
    IPAP-SOFAST-HMQC is presented that allows application on NMR spectrometers equipped
    with cryogenic probes, and fast measurement of one-bond 1H–13C and 1H–15N scalar
    and residual dipolar coupling constants.
article_processing_charge: No
article_type: original
author:
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Ēriks
  full_name: Kupče, Ēriks
  last_name: Kupče
- first_name: Bernhard
  full_name: Brutscher, Bernhard
  last_name: Brutscher
citation:
  ama: Schanda P, Kupče Ē, Brutscher B. SOFAST-HMQC experiments for recording two-dimensional
    deteronuclear correlation spectra of proteins within a few seconds. <i>Journal
    of Biomolecular NMR</i>. 2005;33(4):199-211. doi:<a href="https://doi.org/10.1007/s10858-005-4425-x">10.1007/s10858-005-4425-x</a>
  apa: Schanda, P., Kupče, Ē., &#38; Brutscher, B. (2005). SOFAST-HMQC experiments
    for recording two-dimensional deteronuclear correlation spectra of proteins within
    a few seconds. <i>Journal of Biomolecular NMR</i>. Springer Nature. <a href="https://doi.org/10.1007/s10858-005-4425-x">https://doi.org/10.1007/s10858-005-4425-x</a>
  chicago: Schanda, Paul, Ēriks Kupče, and Bernhard Brutscher. “SOFAST-HMQC Experiments
    for Recording Two-Dimensional Deteronuclear Correlation Spectra of Proteins within
    a Few Seconds.” <i>Journal of Biomolecular NMR</i>. Springer Nature, 2005. <a
    href="https://doi.org/10.1007/s10858-005-4425-x">https://doi.org/10.1007/s10858-005-4425-x</a>.
  ieee: P. Schanda, Ē. Kupče, and B. Brutscher, “SOFAST-HMQC experiments for recording
    two-dimensional deteronuclear correlation spectra of proteins within a few seconds,”
    <i>Journal of Biomolecular NMR</i>, vol. 33, no. 4. Springer Nature, pp. 199–211,
    2005.
  ista: Schanda P, Kupče Ē, Brutscher B. 2005. SOFAST-HMQC experiments for recording
    two-dimensional deteronuclear correlation spectra of proteins within a few seconds.
    Journal of Biomolecular NMR. 33(4), 199–211.
  mla: Schanda, Paul, et al. “SOFAST-HMQC Experiments for Recording Two-Dimensional
    Deteronuclear Correlation Spectra of Proteins within a Few Seconds.” <i>Journal
    of Biomolecular NMR</i>, vol. 33, no. 4, Springer Nature, 2005, pp. 199–211, doi:<a
    href="https://doi.org/10.1007/s10858-005-4425-x">10.1007/s10858-005-4425-x</a>.
  short: P. Schanda, Ē. Kupče, B. Brutscher, Journal of Biomolecular NMR 33 (2005)
    199–211.
date_created: 2020-09-18T10:13:59Z
date_published: 2005-12-01T00:00:00Z
date_updated: 2021-01-12T08:19:38Z
day: '01'
doi: 10.1007/s10858-005-4425-x
extern: '1'
intvolume: '        33'
issue: '4'
keyword:
- Spectroscopy
- Biochemistry
language:
- iso: eng
month: '12'
oa_version: None
page: 199-211
publication: Journal of Biomolecular NMR
publication_identifier:
  issn:
  - 0925-2738
  - 1573-5001
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: SOFAST-HMQC experiments for recording two-dimensional deteronuclear correlation
  spectra of proteins within a few seconds
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 33
year: '2005'
...