--- res: bibo_abstract: - The synaptotrophic hypothesis posits that synapse formation stabilizes dendritic branches, yet this hypothesis has not been causally tested in vivo in the mammalian brain. Presynaptic ligand cerebellin-1 (Cbln1) and postsynaptic receptor GluD2 mediate synaptogenesis between granule cells and Purkinje cells in the molecular layer of the cerebellar cortex. Here we show that sparse but not global knockout of GluD2 causes under-elaboration of Purkinje cell dendrites in the deep molecular layer and overelaboration in the superficial molecular layer. Developmental, overexpression, structure-function, and genetic epistasis analyses indicate that dendrite morphogenesis defects result from competitive synaptogenesis in a Cbln1/GluD2-dependent manner. A generative model of dendritic growth based on competitive synaptogenesis largely recapitulates GluD2 sparse and global knockout phenotypes. Our results support the synaptotrophic hypothesis at initial stages of dendrite development, suggest a second mode in which cumulative synapse formation inhibits further dendrite growth, and highlight the importance of competition in dendrite morphogenesis.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Yukari H. foaf_name: Takeo, Yukari H. foaf_surname: Takeo - foaf_Person: foaf_givenName: S. Andrew foaf_name: Shuster, S. Andrew foaf_surname: Shuster - foaf_Person: foaf_givenName: Linnie foaf_name: Jiang, Linnie foaf_surname: Jiang - foaf_Person: foaf_givenName: Miley foaf_name: Hu, Miley foaf_surname: Hu - foaf_Person: foaf_givenName: David J. foaf_name: Luginbuhl, David J. foaf_surname: Luginbuhl - foaf_Person: foaf_givenName: Thomas foaf_name: Rülicke, Thomas foaf_surname: Rülicke - foaf_Person: foaf_givenName: Ximena foaf_name: Contreras, Ximena foaf_surname: Contreras foaf_workInfoHomepage: http://www.librecat.org/personId=475990FE-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Simon foaf_name: Hippenmeyer, Simon foaf_surname: Hippenmeyer foaf_workInfoHomepage: http://www.librecat.org/personId=37B36620-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2279-1061 - foaf_Person: foaf_givenName: Mark J. foaf_name: Wagner, Mark J. foaf_surname: Wagner - foaf_Person: foaf_givenName: Surya foaf_name: Ganguli, Surya foaf_surname: Ganguli - foaf_Person: foaf_givenName: Liqun foaf_name: Luo, Liqun foaf_surname: Luo bibo_doi: 10.1016/j.neuron.2020.11.028 bibo_issue: '4' bibo_volume: 109 dct_date: 2021^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/1097-4199 dct_language: eng dct_publisher: Elsevier@ dct_title: GluD2- and Cbln1-mediated competitive synaptogenesis shapes the dendritic arbors of cerebellar Purkinje cells@ ...